‘砀山酥梨’叶片中Fer2基因的克隆与表达分析  

Cloning and differential expression analysis of Fer2 gene in leaf of ‘Dangshansuli’ pear

作　　者：钟晨[1] 苏军[1] 汤婷婷[1] 丁伟[1] 朱立武[1] 贾兵[1] 

机构地区：[1]安徽农业大学园艺学院,安徽合肥230036

出　　处：《南京农业大学学报》2013年第5期33-38,共6页Journal of Nanjing Agricultural University

基　　金：国家梨产业技术体系项目(nycytx-29-14)

摘　　要：分别以轻度、中度和重度缺铁的‘砀山酥梨’叶片cDNA为模板,正常叶片为对照,运用RACE技术,克隆Fer2基因的cDNA全长为1 052 bp,编码区828 bp,编码276个氨基酸。采用Protparam、InterProScan和ProtFun 2.2 Server等生物学信息软件分析蛋白质理化性质和结构域特征,结果表明:Fer2与‘翠冠’梨叶片的Fer2蛋白和Fer4蛋白等具有较高的相似性,故命名该基因为PbFer2。推测蛋白质相对分子质量为3 054.3,理论等电点为5.80。通过Real-time PCR技术,分析PbFer2基因表达量,结果表明:轻度缺铁会提高PbFer2的表达量,随着缺铁程度的增加,PbFer2表达量显著下降。The leaves cDNA of＇ Dangshansuli＇ pear （Pyrus bretschneideri ） at different degrees of iron-deficiency were taken as experiment materials. The normal leaves cDNA was taken as CK. The cDNA full length of Fer2 gene was cloned by RACE （rapid- amplification of cDNA ends）method. The results showed that the full length of Fer2 gene was 1 052 bp, and its coding region covered 828 bp and encoded a polypeptide containing 276 amino acids. The physicochemical properties and structures of Fer2 protein were analysed by bioinformatics softwares Protparam, InterProScan and ProtFun 2.2 Server etc. The results showed that Fer2 protein had a higher homology with the protein Fer2 and Fer4 in the leaf of＇ Cuiguan＇ pear and was named as PbFer2 gene. The relative molecular mass of PbFer2 protein in the leaf of＇ Dangshansuli＇ pear was 3 054.3, and the theoretical isoelectric point was 5.80. The PbFer2 gene expression levels were analyzed by Real-time PCR technology. The results showed that the expression level of PbFer2 gene increased in leaf with slight iron-deficiency, but it decreased when the iron-deficiency degree in leaf was increased.

关 键 词：砀山酥梨 缺铁 Fer2基因 克隆 表达分析 

分 类 号：S661.2[农业科学—果树学]