早期大鼠梗阻性脑积水模型MRI及弥散张量成像特征  

MRI and diffusion tensor imaging characteristics in rats models with obstructive hydrocephalus in the early stage

作　　者：李露露[1] 刘鹏飞[1] 刘志兰[1] 李晓娟[1] 周晶[1] 

机构地区：[1]哈尔滨医科大学附属第一医院磁共振室,哈尔滨150001

出　　处：《中华实用诊断与治疗杂志》2013年第10期954-956,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：黑龙江省自然科学基金(D201122)

摘　　要：目的探讨早期大鼠梗阻性脑积水的MRI及弥散张量成像(diffusion tensor imaging,DTI)表现,评估脑白质微观结构的变化。方法雄性Wistar大鼠60只随机分为脑积水组(40只)与对照组(20只),脑积水组向枕大池注入质量分数25%无菌高岭土混悬液0.1mL制作脑积水模型,对照组注入无菌生理盐水0.1mL。于造模后3d及1、2、4周时脑积水组分别取10只大鼠,对照组分别取5只大鼠,腹腔麻醉后2组均行MRI及DTI检查,测量感兴趣区表观弥散系数(apparent diffusion coefficient,ADC)及各向异性(fractional anisotropy,FA)值。结果造模后1、2、4周时,脑积水组侧脑室最大横径与侧脑室面积均高于对照组(P<0.05),随时间增加,脑积水组侧脑室最大横径与面积逐渐增加,不同时间两两比较差异均有统计学意义(P<0.05);造模后3d及1、2、4周时,脑积水组ADC值均高于对照组,FA值均低于对照组(P<0.05),随时间增加,ADC值逐渐增加,FA值逐渐减少,不同时间ADC值与FA值两两比较差异均有统计学意义(P<0.05)。结论 DTI可早期显示脑白质微结构损伤,可解释为轴突损伤和胶质细胞增生,导致功能区受损,产生相应临床症状,掌握治疗时间点至关重要。Objective To explore the MRI and diffusion tensor imaging demonstrations of obstructive hydrocephalus in the early stage, and evaluate microstructural changes of white matter. Methods Sixty male Wistar rats were randomly divided into hydrocephalus group （n=40） and control group （n=20）. Hydrocephalus group was injected 0. 1 mL 25 %sterile kaolin suspension into cisterna magna to establish hydrocephalus models. Control group was injected 0.1 mL sterile saline. The apparent diffusion coefficient （ADC） in region of interest and the fractional anisotropy （FA） were measured on MRI and DTI in 10 rats at the time points of 3 days, 1 week, 2 weeks and 4 weeks after modeling, and in 5 rats in control group at the same time points. Results The maximum diameter and area of lateral ventricle were higher in hydrocephalus group than those in control group 1, 2 and 4 weeks after modeling （P〈0.05）, and increased gradually over time. The maximum diameter and area of lateral ventricle at different time points in pairwise comparisons were significantly different （P〈0.05）. The ADC values were higher and FA values were low at each time point in hydrocephalus group than those in control group （P 〈0.05）. The ADC values increased gradually and FA values decreased gradually over time. ADC and FA values at different time points in pairwise comparisons were significantly different （P〈0.05）. Conclusion DTI reveals microstructure injuries in white matter, which are interpreted as axonal loss and gliosis, resulting in the corresponding functional area damage and clinical symptoms, therefore to get correct the treatment time is essential.

关 键 词：梗阻性脑积水 大鼠 MRI 弥散张量成像 

分 类 号：R651.1[医药卫生—外科学]