ASPP1和ASPP2基因高表达对白藜芦醇诱导乳腺癌MCF-7细胞凋亡能力的影响  

Effects of ASPP1 and ASPP2 on apoptosis in resveratrol-induced MCF-7 breast cancer cells

作　　者：史玉洁[1,2] 谢红建[1,2] 宋晓霞[1,2] 刘秋雨[1,2] 龚智泉[1,2] 吴凯彦[1,2] 

机构地区：[1]郑州大学人民医院 [2]河南省人民医院病理科,郑州450003

出　　处：《中华实用诊断与治疗杂志》2013年第10期976-978,共3页Journal of Chinese Practical Diagnosis and Therapy

摘　　要：目的观察ASPP1和ASPP2基因高表达对白藜芦醇诱导乳腺癌MCF-7细胞凋亡能力的影响。方法采用瞬时转染法将pcDNA3/ASPP1和pcDNA3/ASPP2质粒分别转染入乳腺癌细胞MCF-7中,经G418筛选获得高表达ASPP1和ASPP2的MCF-7阳性克隆细胞株MCF-7/ASPP1与MCF-7/ASPP2。MCF-7、MCF-7/ASPP1与MCF-7/ASPP2细胞分别加入0、25、50μmol/L白藜芦醇,采用MTT法检测细胞增殖情况,RT-PCT法检测细胞p53下游基因bax与p21mRNA表达情况;3种细胞分别加入0、100、200μmol/L白藜芦醇,采用ELISA法检测细胞凋亡率。结果 MTT结果显示,25、50μmol/L白藜芦醇处理后,MCF-7/ASPP1与MCF-7/ASPP2细胞存活细胞吸光度值均明显低于MCF-7细胞(P<0.05);RT-PCR结果显示,25、50μmol/L白藜芦醇处理后,MCF-7/ASPP1与MCF-7/ASPP2细胞中Bax和p21mRNA表达水平高于MCF-7细胞;细胞凋亡试验结果显示,100、200μmol/L白藜芦醇处理后,MCF-7/ASPP1与MCF-7/ASPP2细胞凋亡细胞吸光度值均明显高于MCF-7细胞(P<0.05)。结论 ASPP1和ASPP2高表达可增强乳腺癌细胞对白藜芦醇的敏感性,且随浓度增高而敏感性增强。Objective To observe the effects of the over expressions of ASPP1 and ASPP2 on the apoptosis ability in resveratrol induced MCF 7 breast cancer cells. Methods pcDNA3/ASPP1 and pcDNA3/ASPP2 were transfected into MCF 7 cells by Fugen 6. Positive clone cells MCF-7/ASPP1 and MCF-7/ASPP2 were obtained by persistent G418 selection. MCF-7, MCF-7/ASPP1 and MCF 7/ASPP2 cells were treated with 0, 25 and 50 μmol/L resveratrol, respectively, and were detected apoptosis cells by using MTT assay. RT PCR was applied to detect the expression of apoptosis associated protein as Bax and p21 mRNA. MCF-7, MCF 7/ASPP1 and MCF 7/ASPP2 cells were treated with 0, 100 and 200 μmol/L resveratrol, respectively, and were detected the apoptosis rates by using ELISA technique. Results MTT OD values of survival cells were lower in MCF-7/ASPP1 and MCF-7/ASPP2 cells than those in MCF 7 cells after the treatment with 25 and 50 μmol/L resveratrol, which showed significant differences （P〈0.05）. RT-PCR results showed that after the treatment with 25 and 50 μmol/L resveratrol, the levels of Bax and p21 mRNA were higher in MCF 7/ASPP1 and MCF-7/ASPP2 cells than those in MCF-7 cells. Apoptosis OD values of death cells were higher in MCF 7/ASPP1 and MCF-7/ASPP2 cells than those in MCF 7 cells after the treatment with 100 and 200 μmol/L resveratrol, showing significant differences （P〈0.05）. Conclusion The over expressions of ASPP1 and ASPP2 improve the sensitivity of MCF-7 cells to resveratrol, and the sensitivity is increased when the concentration is high.

关 键 词：乳腺癌 白藜芦醇 ASPP1 ASPP2 凋亡 

分 类 号：R285.5[医药卫生—中药学]