正常小鼠冠状动脉的超声成像技术分析  

Analysis of coronary artery imaging in healthy mice with echocardiography

作　　者：杨海明[1] 杜忠东[1] 上官文[1] 张艳兰[1] 董伟[2] 宋铭晶[2] 郑枝兰 

机构地区：[1]首都医科大学附属北京儿童医院,北京100045 [2]中国医学科学院医学实验动物研究所,北京100021 [3]美中互利医疗有限公司,北京100045

出　　处：《中国比较医学杂志》2013年第9期27-30,共4页Chinese Journal of Comparative Medicine

基　　金：国家自然基金面上项目(81274109;30973238);北京自然科学基金B类/北京教育委员会重大科研项目(KZ201010025024);北京市教育委员会科技创新平台项目(PXM2011_014226_07_000085);北京市卫生系统高层次卫生技术人才培养计划项目(2009-3-38)

摘　　要：目的探讨利用高频小动物心脏超声对C57BL/6小鼠冠状动脉进行评价的可行性,为小鼠冠状动脉相关疾病动物模型的制备及其功能评价提供依据。方法采用Vevo 770型高分辨小动物超声仪,频率30 mHz的宽频探头,对20只健康C57BL/6小鼠于4、8和12周龄时冠状动脉的情况进行观察。测定和分析不同周龄小鼠冠状动脉内径值的变化。结果全部20只小鼠超声均成功检测到冠状动脉。超声心动图显示小鼠4周龄时左冠状动脉主干内径检测值为0.36±0.02 mm,右冠状动脉主干内径值为0.29±0.03 mm;8周龄时左冠状动脉主干内径值为0.38±0.06 mm,右冠状动脉主干内径值为0.37±0.02(mm);12周龄时左冠状动脉主干内径值为0.38±0.02 mm,右冠状动脉主干内径值为0.39±0.03 mm。结论利用高频小动物心脏超声可获取正常小鼠清晰的冠状动脉图像,并能准确反映小鼠冠状动脉内径值动态变化。为小鼠冠状动脉疾病模型的制备及其功能评价提供依据。Objective To assess the feasibility of coronary artery in C57BL/6 mice With a high frequency ultrasound system and to provide evidence for preparation the murine model of KD. Methods Totally 20 mice were chosen and Visualsonics Vevo 770 UBM was used in this study. On weeks 4, 8 and 12, the developmental changes and dimensions of coronary artery of the mice were measured by echocardiography. Results By echocardiography, the present study successfully detected coronary artery diameter in the routine model of KD. On week 4, the diameter of left coronary artery in the mice was 0. 32 ~0. 14 mm, and the diameter of right coronary artery was 0.37 ~0. 02 mm, On week 8, the diameter of left coronary artery in the mice was O. 38 ~ 0. 06 ram, and the diameter of right coronary artery was 0. 37 ~ 0. 02 mm; on week 12, the diameter of left coronary artery in the mice was 0. 38 ~ 0. 02 ram, and the diameter of right coronary artery was 0. 39 ＋0.03 mm. Conclusions The the diameter of coronary artery in the mice coronary arteries disease. present study successfully could be measured which detected coronary arteries by way be benefit for the study echocardiography, and in the murine model of

关 键 词：超声心动图 小鼠 冠状动脉 

分 类 号：R332[医药卫生—人体生理学]