剂量精确的大鼠无创气管滴注方法  

A high accuracy method for non-invasive intratracheal instillation in rats

作　　者：彭康琳[1] 周玉庆[2] 周正适[3] 

机构地区：[1]中南大学湘雅二医院心胸外科,长沙410000 [2]中南大学湘雅三医院心内科,长沙410000 [3]中南大学湘雅医学院实验动物学部,长沙410000

出　　处：《中国比较医学杂志》2013年第9期61-63,30,共4页Chinese Journal of Comparative Medicine

摘　　要：目的建立一种滴注剂量精确的大鼠无创气管滴注方法。方法选用一次性使用静脉输液针的胶管制作套管,透射光插管法行大鼠气管插管,微量进样器平头针经由套管插入气管进行滴注。滴注液体为生理盐水,滴注剂量5～200μL,精确度为1μL。结果在体重200～350 g的10只大鼠上共完成50次气管滴注操作,实验过程中和实验之后大鼠均无异常表现。结论套管微量进样器法具有无创、精确、安全的特点,可广泛应用于大鼠经气管给药或染毒的各类研究。Objective To develop a high accuracy method for non-invasive intratracheal instillation in rats. Methods The plastic catheter of disposable venous infusion needle was cut into a cannula, which was then intubated by transillumination. A microsyringe was introduced into the rat trachea through the cannula before the intratracheal instillation of saline solution was performed. The instilled volume ranged from 5 ~L to 200 ~L and the accuracy is 1 ~L. Results The intratracheal instillation operation was performed successfully for 50 times in 10 rats weighing 200 ~ 350g. The general health status was not affected during or after the operation in the rats. Conclusion The method developed in our study for intratracheal instillation is characterized by non-invasion, accuracy and safety,which could be widely used in intratracheal drug administration or poisoning in rats.

关 键 词：大鼠 气管滴注 方法 

分 类 号：R332[医药卫生—人体生理学]