七氟醚预处理对脑缺血再灌注损伤后含核转录因子κB反应元件相关蛋白的影响  

The Effect of Sevoflurane Preconditioning on Proteins with Regulatory Element κB against Cerebral Ischemia and Reperfusion

作　　者：余琼[1] 梁伟民[1,2,3] 

机构地区：[1]复旦大学附属华山医院麻醉科,200040 [2]上海市静安区中心医院 [3]华山医院静安分院麻醉科,200040

出　　处：《中国临床神经科学》2013年第5期504-512,共9页Chinese Journal of Clinical Neurosciences

基　　金：上海市卫生局青年科研基金项目(编号:20114y128);国家自然科学基金青年科学基金项目(编号:81200937)

摘　　要：目的探讨七氟醚预处理对大鼠脑缺血再灌注损伤后含核转录因子κB(NF-κB)反应元件的蛋白细胞黏附分子[细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)]、基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)的影响。方法雄性SD大鼠随机分为假手术组、缺血组和七氟醚预处理组。采用线栓法制备大脑中动脉栓塞大鼠模型。Western blot检测缺血再灌注6、24、48和72 h后缺血侧ICAM-1、VCAM-1、MMP-2和MMP-9表达。伊文思蓝(EB)染色观察血脑屏障的通透性。结果七氟醚预处理组缺血侧脑组织中EB含量显著低于缺血组(P<0.01)。缺血组各再灌注时间点ICAM-1、VCAM-1、MMP-2和MMP-9表达均显著高于七氟醚预处理组。结论七氟醚预处理可能通过抑制再灌注损伤后含NF-κB反应元件的ICAM-1、VCAM-1、MMP-2和MMP-9的上调起神经保护作用。Aim To study the effect of sevoflurane preconditioning on proteins, including cellular adhesion molecules(CAMs) and matrix metalloproteinases(MMPs), with regulatory element κB after cerebral ischemia and reperfusion(I/R) in rats. Methods Transient focal cerebral I/R was induced by using 60 min of middle cerebral artery occlusion(MCAO) in adult male Sprague-Dawley rats. The animals were exposed for 30 min per day on 4 consecutive days to ambient air(sham and vehicle group) or to 1.2% sevoflurane(sevo-pre group), and then subjected to MCAO 24 hours later. Blood-brain barriers(BBB) integrity was detected by Evans blue extravasation at 48 hours after ischemia. Biochemical measurements for intracellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1), MMP-2 and MMP-9 were conducted at 6 to 72 hours after I/R. Results Evans blue extravasation showed that the total EB content was lower in sevopre group than in vehicle group(P<0.01). Ischemia-induced increases in ICAM-1, VCAM-1, MMP-2 andMMP-9 were significantly inhibited in sevo-pre group. Conclusion Repeated preconditioning with sevoflurane confers beneficial effect on ischemic brain injury, partly by suppressing the elevation of proteins with regulatory element κB, such as ICAM-1, VCAM-1, MMP-2 and MMP-9.

关 键 词：七氟醚 神经保护 细胞黏附分子 血管细胞黏附分子 基质金属蛋白酶 核转录因子ΚB 

分 类 号：R416.2[医药卫生—临床医学]