Feasibility of enhanced phytoextraction of Zn contaminated soil with Zn mobilizing and plant growth promoting endophytic bacteria  

促植物生长内生细菌强化植物修复锌污染土壤(英文)

作　　者：龙新宪[1,2] 陈雪梅[1,2] 黄焕忠[3] 卫泽斌[1,2] 吴启堂[1,2] 

机构地区：[1]华南农业大学资源环境学院,广州510642 [2]华南农业大学广东省教育厅土壤环境与废物利用重点实验室,广州510642 [3]香港浸会大学生物系

出　　处：《Transactions of Nonferrous Metals Society of China》2013年第8期2389-2396,共8页中国有色金属学报（英文版）

基　　金：Project(40973055) supported by the National Natural Science Foundation of China;Project(U0833004) supported by the NSFC-Guangdong Joint Foundation of China

摘　　要：Three bacterial endophytes of Sedum alfredii, VI8L2, II8L4 and VI8R2, were examined for promoting soil Zn bioavailability and Zn accumulation in S. alfredii. Results showed that three strains were re-introduced into S. alfredii rhizosphere soils under Zn stress and resulted in better plant growth, as roots biomass increased from 80% to 525% and shoot biomass from 11% to 47% compared with the uninoculated ones. Strains IVsLz, II8L4 and IVsR2 significantly increased shoot and root Zn concentrations in the ZnCO3 contaminated soil. Inoculation with strain IVsL2 resulted in 44% and 39% higher shoot and root Zn concentrations, while strain IV8R2 significantly decreased shoot Zn concentration in the Zn3（PO4）2 contaminated soils. In the aged contaminated soil, isolates IVsL2, IIsL4 and IVsR2 significantly increased root Zn concentration, but decreased shoot Zn concentration of Sedum alfredii. It suggested that endophytes might be used for enhancing phytoextraction efficiency.以超积累植物东南景天为材料,采用盆栽试验探讨了接种内生菌Ⅵ8L2、Ⅱ8L4和Ⅵ8R2对土壤Zn有效性、植物生长和吸收积累锌的影响。结果表明,接种内生菌Ⅵ8L2、Ⅱ8L4和Ⅵ8R2能够促进东南景天在Zn污染土壤中的生长,其根系和地上部的生物量分别比对照增加了80%～525%和11%～47%。在人工ZnCO3污染土壤中,接种菌株Ⅵ8L2、Ⅱ8L4和Ⅵ8R2显著增加了东南景天根系和地上部的Zn含量;在人工Zn3(PO4)2污染土壤中,菌株Ⅵ8L2使东南景天地上部和根系的Zn含量分别比对照增加了44%和39%,但是菌株Ⅳ8R2显著降低了东南景天地上部的Zn含量;在长期被酸性废水污染水稻土壤中,接种菌株Ⅵ8L2、Ⅱ8L4和Ⅵ8R2显著增加了东南景天根系的Zn含量,但降低了地上部的Zn含量。这表明金属抗性促生细菌可用于强化植物修复重金属污染土壤。

关 键 词：endophytic bacteria ZN BIOAVAILABILITY PHYTOREMEDIATION Sedum alfredii 

分 类 号：X53[环境科学与工程—环境工程]