蒙药大栀子普通粉与超微粉高效液相色谱指纹图谱的比较  

Comparative Study on HPLC Fingerprint of Ordinary Powder and Ultrafine Powder for Gardenia jasminoides f. longicarpa

作　　者：鞠爱华[1] 周凯 张静 蔡丽娟[1] 宋萍萍[1] 

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010059 [2]内蒙古食品药品检验所,内蒙古呼和浩特010059

出　　处：《中药材》2013年第7期1072-1075,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技部支撑计划蒙药产业化共性关键技术研究项目(2012BAI28B01)

摘　　要：目的:建立大栀子药材HPLC指纹图谱研究方法,并比较大栀子药材普通粉与超微粉指纹图谱的差异,以揭示超微粉碎对大栀子化学成分溶出的影响。方法:采用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,乙腈-0.4%磷酸为流动相进行梯度洗脱,流速1.0 mL/min,0～40 min检测波长为240 nm,40 min后为440 nm。结果:通过对10批大栀子药材指纹图谱的分析,确立了23个共有峰,10批大栀子药材的相似度评价结果均大于0.9;大栀子超微粉碎后,化学成分种类数目并未发生改变但其主要化学成分的含量明显提高。结论:该方法准确可靠,重现性好,可作为大栀子质量控制的有效方法。超微粉碎能促进大栀子化学成分的溶出。Objective：To establish an HPLC fingerprint of Gardenia jasminoicles f. longicarpa and compare the differences between its ordinary powder and ultrafine powder. Methods ：The analysis was carried out on a Kromasil Cls （250 mm ×4.6mm,5μm）column with gradient elutiou of acetonitrile-0.4% phosphoric acid at the flow rate of 1.0 mL/min. The wavelength was 240 nm during 0 - 40 rain and 440 nm during 40 - 80 min. Results ： HPLC fingerprint of Gardenia jasminoides f. longicarpa was established, 23 common peaks were identified, and the similarity of 10 samples was greater than 0. 9. Ultrafine grinding did not change the types and number of chemical compositions, but it obviously increased the content of main chemical compositions. Conclusion ： The HPLC fingerprint is accurate,reliable and repeatable, which can be used for quality control of Gardenia jasminoides f. longicarpa. Uhrafine grinding can stimulate the release of chemical components of Gardenia jasminoides f. longicarpa.

关 键 词：蒙药大栀子 超微粉 HPLC指纹图谱 

分 类 号：R282.5[医药卫生—中药学]