百合药材UPLC特征图谱及特征峰QTOF-MS分析研究  

Study on UPLC Specific Chromatogram of Lily and Its Specific Peaks Compositions Analysis by QTOF-MS

作　　者：聂慧[1,2] 严辉[2] 钱大玮[2] 段金廒[2] 欧阳臻[1] 钱叶飞[2] 管汉亮[2] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]南京中医药大学/江苏省方剂高技术研究重点实验室,江苏南京210046

出　　处：《中药材》2013年第7期1087-1092,共6页Journal of Chinese Medicinal Materials

基　　金：国家中医药管理局行业科研专项(201107009)

摘　　要：目的:建立百合药材超高效液相色谱(UPLC)特征图谱,并采用电喷雾离子源-四极杆-飞行时间质谱(ESI-QTOF-MS)对其特征峰进行定性研究。方法:采用ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,以乙腈-0.1%甲酸为流动相梯度洗脱,流速0.4 mL/min,检测波长320 nm,柱温35℃。质谱检测采用负离子模式;电压3.0 kV;离子源温度120℃;雾化温度350℃;雾化气600 L/h。结果:获得百合药材UPLC特征图谱,共有峰19个,鉴定出12个酚性甘油酯类成分,其中6个通过对照品对照确认,6个通过质谱信息推测。结论:建立的UPLC-UV特征图谱可用于百合药材质量评价,为全面控制其质量提供了科学依据。Objective：To establish the UPLC specific chromatogram of Lily and analyze the specific peaks compositions by ESI- QTOF-MS. Methods：The samples were conducted by ACQUITY UPLC BEH C18 Column（2. 1 mm×100mm,1.7μm）and eluted with acetonitrile and 0. 1% formic acid at the flow rate of 0. 4 mL/min. The detection wavelength was set at 320 nm and column temperature was 35 ℃. Negative ion mode was chosen for qualitative analysis. The capillary voltage was set at 3.0 kV. The nebulization gas was set to 600 L/h at 350 ℃, and the source temperature was 120 ℃. Results：The specific chromatogram of Lily was obtained. There were 19 common peaks. Twelve phenylpropenoid glycerides compositions were identified. Among them, 6 compositions were identified by comparison with the reference substances and others were identified by MS and MS2 data. Conclusion：UPLC specific chromatogram can be used for the quality evaluation of Lily, giving support to quality control comprehensively.

关 键 词：百合 UPLC特征图谱 特征峰 QTOF—MS 

分 类 号：R284.2[医药卫生—中药学]