超临界CO_2流体萃取板蓝根中(R,S)-告依春的工艺  

The Extraction Technology of Epigoitri from Isatidis Radix by Supercritical CO_2 Fluid

作　　者：年四辉[1] 柳春燕[1] 刘丽敏[2] 杨丽芬[1] 

机构地区：[1]皖南医学院药学系,安徽芜湖241002 [2]安徽中医药高等专科学校药学系,安徽芜湖241000

出　　处：《中药材》2013年第7期1163-1166,共4页Journal of Chinese Medicinal Materials

基　　金：皖南医学院中青年科研基金(WK200812);安徽省教育厅项目(KJ2009B104Z);芜湖市科技局重点项目(2009卫1-5)

摘　　要：目的:优化用超临界CO2萃取板蓝根中(R,S)-告依春的工艺条件。方法:采用单因素试验和正交试验研究了压力、温度、时间、夹带剂浓度、夹带剂用量对板蓝根中(R,S)-告依春萃取质量的影响,确定最佳工艺条件。结果:超临界CO2萃取板蓝根中(R,S)-告依春的最佳条件为:压力20 MPa,温度50℃,时间2 h,夹带剂乙醇浓度100%,夹带剂体积80 mL,在此优化条件下,(R,S)-告依春在提取物中的含量可达到38.63%。结论:该工艺简单、快速,可用于板蓝根中(R,S)-告依春的提取。Objective：To study the extraction technology of epigoitri from Isatidis Radix by supercritical CO2 fluid. Methods：The effects of pressure, temperature, time, concentration and dosage of alcohol were studied by single factor analysis and orthogonal test. Results：The optimized conditions were as follows： The pressure was 20 MPs, the temperature was 50 ℃, the time was 2 h, concentration of alcohol was 100% , dosage was 80 mL. The content of epigoitri in the extract could reach 38.63% under the above conditions. Conclusion ： This method is simple, rapid and it is suitable for the extraction of epigoitri from Isatidis Radix.

关 键 词：超临界 板蓝根 （R S）-告依春 

分 类 号：R286.1[医药卫生—中药学]