Hemoglobin-glucose oxidase catalyzed polymerization of aniline:Electrochemical study and application  

作　　者：Hong-Fang Zhang Jian-Bin Zheng Rui-Xiao Liu 

机构地区：[1]Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecular Chemistry,College of Chemistry and Materials Science,Northwest University [2]Institute of Analytical Science,Northwest University

出　　处：《Chinese Chemical Letters》2013年第10期934-936,共3页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.20905061)

摘　　要：A new method for the formation of electroactive polyaniline （PANI） biocatalyzed by hemoglobin coupled with glucose oxidase in neutral medium on the polystyrene nanospheres （PS） modified glassy carbon electrode, was investigated. The bio-polymerized PANI formed on the PS was confirmed by the obvious increase of the diameter of the particles on the scanning electron microscopy image. The cyclic voltammetric behavior of the PANI was also investigated. PANI produced an oxidative peak at 0.28 V and a reductive peak at 0.23 V. Based on the glucose-dependent bio-polymerization, a new electrochemical protocol for the estimation of glucose was developed. The square wave voltammetric response of PANI deposited on the modified electrode increased linearly with glucose concentration in the range of 0.1- 10.0 μmol/L. The efficient performance of hemoglobin-oxidase biocatalyzed polymerization of aniline provides a new concept for the synthesis of nanomaterials, and a general protocol for the development of the biosensors.A new method for the formation of electroactive polyaniline （PANI） biocatalyzed by hemoglobin coupled with glucose oxidase in neutral medium on the polystyrene nanospheres （PS） modified glassy carbon electrode, was investigated. The bio-polymerized PANI formed on the PS was confirmed by the obvious increase of the diameter of the particles on the scanning electron microscopy image. The cyclic voltammetric behavior of the PANI was also investigated. PANI produced an oxidative peak at 0.28 V and a reductive peak at 0.23 V. Based on the glucose-dependent bio-polymerization, a new electrochemical protocol for the estimation of glucose was developed. The square wave voltammetric response of PANI deposited on the modified electrode increased linearly with glucose concentration in the range of 0.1- 10.0 μmol/L. The efficient performance of hemoglobin-oxidase biocatalyzed polymerization of aniline provides a new concept for the synthesis of nanomaterials, and a general protocol for the development of the biosensors.

关 键 词：Biocatalysis Polyanitine Electrochemical Glucose 

分 类 号：O631.5[理学—高分子化学]