An immunochromatographic assay for rapid and simultaneous detection of levonorgestrel and methylprednisolone in water samples  

作　　者：Hua-Fang Chang Jun-Qing Wang Bin Wang An-Ping Deng 

机构地区：[1]College of Chemistry,Chemical Engineering & Materials Science,Soochow University [2]College of Chemistry,Sichuan University

出　　处：《Chinese Chemical Letters》2013年第10期937-940,共4页中国化学快报（英文版）

基　　金：fnancial support from the National Natural Science Foundation of China(No.21175097)

摘　　要：Synthetic contraceptive levonorgestrel （LNG） and glucocorticoid methylprednisolone （MP） residues are eventually discarded to environmental water system and function as environmental hormones, displaying potential risk to humans and ecosystems, thus there is an urgent need for fast, sensitive and simultaneous detection of these compounds in water samples. In this study, a competitive immunochromatographic assay （ICA） using colloidal gold-labeled polyclonal antibodies as probes for rapid and simultaneous detection of LNG and MP in water samples was developed. The visual detection limits of LNG and MP in water samples were 10 ng/mL. The detection process could be completed within 10 min. There was no cross-reactivity of the ICA with other seven compounds. The strips could be stored at 4 ℃ for 10 weeks without significant loss of activity. The assay is a suitable tool for rapid and semi- quantitative detection of LNG and MP in water samples on site.Synthetic contraceptive levonorgestrel （LNG） and glucocorticoid methylprednisolone （MP） residues are eventually discarded to environmental water system and function as environmental hormones, displaying potential risk to humans and ecosystems, thus there is an urgent need for fast, sensitive and simultaneous detection of these compounds in water samples. In this study, a competitive immunochromatographic assay （ICA） using colloidal gold-labeled polyclonal antibodies as probes for rapid and simultaneous detection of LNG and MP in water samples was developed. The visual detection limits of LNG and MP in water samples were 10 ng/mL. The detection process could be completed within 10 min. There was no cross-reactivity of the ICA with other seven compounds. The strips could be stored at 4 ℃ for 10 weeks without significant loss of activity. The assay is a suitable tool for rapid and semi- quantitative detection of LNG and MP in water samples on site.

关 键 词：Immunochromatographic assay kevonorgestrel Methylprednisolone Colloidal gold 

分 类 号：R927[医药卫生—药学]