兔神经端侧吻合再生轴突荧光素双标记研究  

Origin of regenerative axon in receptive nerves after end-to-side neurorrhaphy in rabbits

作　　者：曲辉[1] 陈绍宗[1] 刘明锁[1] 李跃军[1] 李学拥[1] 

机构地区：[1]第四军医大学唐都医院整形外科,陕西西安710038

出　　处：《第四军医大学学报》2000年第12期1501-1502,共2页Journal of the Fourth Military Medical University

摘　　要：目的　研究周围神经端侧吻合后受神经内再生轴突的来源 .方法　采用兔两侧耳大神经端侧吻合模型 ,于术后 8wk用 fast blue(FB)和 nuclear yellow(NY)作荧光素逆行追踪双标记 ,荧光显微镜下观察、计数背根节内标记的神经元 .结果　右侧 C2 - 3背根节内大多数神经元被 FB单标记 ,部分被NY单标记 ,少数为 FB/ NY双标记 .FB/ NY双标记细胞多为直径 5 0 μm以下的中、小型细胞 .结论　神经端侧吻合后 8wk。AIM To study the origin of the regenerative axon in the receptive nerve after end-to-side neurorrhaphy on periphery nerves. METHODS Model of the New Zealand rabbit greater auricular nerve(GAN) was used: The left GAN was dissociated and sectioned. After a 2 mm epineurium (epineurial window) was removed in the right GAN, the distal end of the left GAN was sutured to the lateral face of the right GAN. After 8 weeks, the technique of double-labeled tracing with fluorescence made up of fast blue (FB) and nuclear yellow (NY) was used and the labeled neurons in the dorsal root ganglion (DRG) were observed under the fluorescence microscope and counted. RESULTS Most neurons in the right C_ 2-3 DRG were single-labeled with FB; some were single-labeled with NY and extremely few were double-labeled with FB/NY. Most neurons double-labeled with FB/NY were small or medium in size which had diameters below 50 μm. CONCLUSION Eight weeks after end-to-side neurorrhaphy, axons or their collateral sprouts of some donor nerves can regenerate into receptive nerves.

关 键 词：神经再生 端侧吻合 荧光素 逆行追踪 

分 类 号：R651.3[医药卫生—外科学]