运脾复方对幼龄大鼠厌食症模型胃肠激素的影响  

Effects of yunpi complex on gut hormones in juvenile rat model with anorexia

作　　者：杜永平[1] 张月萍[2] 史健[1] 汪静[3] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710033 [2]第四军医大学西京医院小儿科,陕西西安710033 [3]第四军医大学西京医院核医学科,陕西西安710033

出　　处：《第四军医大学学报》2000年第12期1543-1545,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助课题(39670896)

摘　　要：目的　研究运脾复方对小儿厌食症动物模型胃肠激素的影响 ,探讨运脾复方治疗小儿厌食症的机制 .方法　模拟小儿厌食症的主要病因制作幼龄大鼠厌食模型 ,用运脾复方儿宝颗粒灌胃 ,用放免分析法测定模型下丘脑、胃窦部和外周血 CCK- 8和 β- EP的含量 .结果　模型动物下丘脑和血浆CCK- 8浓度 (12 3± 32 ) pmol· g- 1 和 (5 0 7± 113) ng· L- 1 ,高于对照组的 (89± 18) pmol· g- 1和 (2 5 4± 95 ) ng· L- 1 (P<0 .0 5 ) ;模型动物胃窦部和血浆β- EP浓度 (1.4± 0 .6 )μg· g- 1和 (6 .3± 1.7) ng· L- 1 ,低于对照组的 (6 .3± 1.4) μg· g- 1 和(10 .2± 4.9) ng· L- 1 (P<0 .0 5 ) .运脾复方治疗组 CCK- 8恢复正常 ,β- EP浓度显著增加 .结论　儿宝颗粒能明显抑制模型动物中枢和外周 CCK- 8分泌 ,促进 β- EP分泌 。AIM To study the effects of yunpi complex erbao granules on gut hormones in juvenile animal model with anorexia. METHODS Model was established by imitating the major cause of children anorexia. Cholocystokinin-octapeptide (CCK-8) and beta-endorphin(β-EP) concentration in hypothalamus, antrum pyloricum and plasma of animals were examined by radioimmunoassay.RESULTS CCK-8 concentration in hypothalumus and plasma of the model group rose up to (123±32)pmol· g -1 and (507±113)ng·L -1 which were more than control group: (89±18)pmol· g -1 and (254±95)ng·L -1(P<0.05). Compared with (6.3±1.4)μg·g -1 and (10.2±4.9)ng·L -1 of control group,β-EP concentration in antrum pyloricum and plasma of the model group declined to (1.4±0.6)μg·g -1 and (6.3±1.7)ng·L -1(P<0.05). These things got better in two treatment groups. CONCLUSION Yunpi complex was effective on decreasing CCK-8 and increasing β-EP secretion in the model.

关 键 词：厌食 动物模型 儿宝颗粒 八肽胆囊收缩素 Β-内啡肽 

分 类 号：R285.5[医药卫生—中药学] R272.605[医药卫生—中医学]