胶质细胞源性神经营养因子对脊髓前角神经元的保护作用  

Protective effect of glial cell line derived neurotrophic factor on spinal motoneurons after sciatic nerve injury in rats

作　　者：陈哲宇[1] 黄爱军[1] 路长林[1] 何成[1] 

机构地区：[1]上海第二军医大学神经生物学教研室,200433

出　　处：《中华创伤杂志》2000年第12期723-726,共4页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目!(30 0 0 0 0 48)

摘　　要：目的　探讨胶质细胞源性神经营养因子 (GDNF)对坐骨神经切断后引起的脊髓前角运动神经元退行性变的影响。　方法　以硅胶管套接切断的大鼠坐骨神经为实验模型 ,2 4只大鼠随机分为 2组 ,治疗组硅胶管内注射GDNF ,对照组注射等渗盐水 ,术后 2周观察大鼠后肢运动功能 ,并进行脊髓切片的尼氏染色、酸性磷酸酶 (ACP)组织化学染色及原位末端标记 (TUNEL)法染色。　结果　与对照组相比 ,治疗组后肢运动功能明显改善 ;脊髓前角运动神经元丢失减少 ,其存活率由 85 .3 %上升至 95 .8% (P <0 .0 1) ;ACP变化幅度降低 ,ACP染色阳性面积百分率由 376 %降低至 2 5 2 % (P <0 .0 1) ;TUNEL阳性细胞数由 34.8减少至 13 .2 (P <0 .0 1)。Objective To investigate the effect of glial cell line derived neurotrophic factor(GDNF) on spinal motoneurons following sciatic nerve axotomy. Methods Rats with the right sciatic nerve transected were used as models. Twenty four rats were randomized into a treated group (GDNF was injected into the silica-gel tube) and a control group (Isotonic saline was injected). After silicone tubulization of sciatic nerve in adult rats, normal saline was injected into silicone chamber of the control group, and GDNF solution was injected in the treated group during operation. Two weeks after operation, hindlimb motor functions were measured and spinal sections were stained by Nissl, acid phosphatase(ACP) enzyme histochemistry and TUNEL methods. Results Compared with the control group, the hindlimb motor function was improved, the percentage of the survived spinal motoneuron was increased from 85.3% to 95.8%( P <0.01), the percentage of AC P stained area was significantly decreased from 376% to 252%( P <0.01)and TUNEL positive cells were reduced from 34.8 to 13.2 ( P <0.01)in the treated group. Conclusions GDNF could protect the degenerated spinal motoneuron after sciatic nerve axotomy.

关 键 词：神经损伤 GDNF 脊髓前角神经元 保护作用 

分 类 号：R33[医药卫生—人体生理学]