北冬虫夏草组织分离及复壮菌种的试验研究  

Experimental Research on Tissue Isolation and Strain Rejuvenation of Cordyceps militaris

作　　者：胡中娥[1] 钟国祥[1] 沈爱喜[1] 张诚[1] 魏云辉[1] 陈庆隆[1] 谢晟[1] 陈浩[2] 

机构地区：[1]江西省农业科学院农业应用微生物研究所,江西南昌330200 [2]四川省原子能研究院,四川成都610000

出　　处：《江西农业学报》2013年第10期124-125,134,共3页Acta Agriculturae Jiangxi

基　　金：公益性行业(农业)科研专项(201103007;201203072;201303080-1)

摘　　要：试验结果表明:采用组织分离+复壮的技术手段可明显提高北冬虫夏草菌种菌丝的生长活力,复壮后菌丝日平均生长速率达2.65mm/d,液体培养7d后净增干物质重0.65g;复壮后菌种的出草性状得到了明显改善,平均每瓶鲜子实体产量可达27.2g。The experimental results indicated that the technological means of tissue isolation plus strain rejuvenation could obvi- ously strengthen the growth capacity of the mycelia of Cordyceps militaris. After the strain rejuvenation, the daily average growth rate of mycelium could reach 2.65 mm/d, and its dry matter weight had a net increase of 0.65 g after 7 days＇ liquid culture. After ap- plying the rejuvenation approach, the production traits of the strain were markedly improved, and the average fresh weight of the fruiting body could reach 27.5 g per bottle.

关 键 词：北冬虫夏草 组织分离 复壮 菌种 

分 类 号：S567.35[农业科学—中草药栽培]