HPLC测定胡黄连中胡黄连甙-Ⅱ的含量  

Determination of Picroside-ⅡContained in Picrorhiza scrophulariiflora by HPLC

作　　者：王菁[1] 张朝晖 戴永健[2] 吴如金[1] 

机构地区：[1]中国药科大学药物分析教研室,南京210009 [2]南京军医学院,南京210099

出　　处：《中国药科大学学报》2000年第5期368-370,共3页Journal of China Pharmaceutical University

摘　　要：利用高效液相色谱法建立了测定胡黄连药材中胡黄连甙 Ⅱ的测定方法 ,并用该方法比较了印度胡黄连和西藏胡黄连中胡黄连甙 Ⅱ的含量差别。在选定的实验条件下 ,胡黄连甙 Ⅱ在 4.8～ 2 4μg/ml范围内 ,加入量与峰面积呈良好的线性关系 .该组分的回收率分别是 ,印度胡黄连 99.5 % ,RSD为 2 .1% (n =5 ) ;西藏胡黄连 98.7% ,RSD为 1.8% (n =5 ) ,为制定其质量标准提供了参考依据。A HPLC method was established for the determination of Picroside-Ⅱand the difference of the amount of Picroside-Ⅱcontained in \%Picrorhiza kurroa\% and \%Picrorhiza scrophulariiflora\% were compared. Under the selected experimental condition, the calibration of Picroside-Ⅱ was within the linear range of 4.8～24 μg/ml, the rate of recovery of \%Picrorhiza kurroa\% was 99.5%, RSD was 2.1%, \%Picrorhiza scrophulariiflora\% was 98.7%, and RSD was 1.8% ( n =5), which provided basis for developing quality control method.

关 键 词：胡黄连 胡黄连甙-Ⅱ 含量测定 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]