洋水仙MYB基因的克隆与序列分析  

Cloning and sequence analysis of MYB gene from Narcissus pseudonarcissus

作　　者：郑益平[1] 温超[1] 曾黎辉[1] 

机构地区：[1]福建农林大学园艺学院/福建农林大学园艺植物遗传育种研究所,福建福州350002

出　　处：《亚热带农业研究》2013年第3期202-206,共5页Subtropical Agriculture Research

基　　金：福建省自然科学基金项目(2011J01084)

摘　　要：根据已知MYB基因设计引物,以洋水仙粉红魅力花瓣总RNA逆转录的产物为模板进行PCR扩增,获得一个长为333 bp基因片段,命名为NpMYB,并通过3'RACE技术获得该基因的3'端序列。NpMYB基因的氨基酸序列与丹参、矮牵牛、拟南芥、白花紫露草和石斛兰的相似度分别为77%、74%、67%、68%和68%,NpMYB存在完整R2R3重复结构域,属于典型的R2R3-MYB转录因子基因。The conserved region sequence of a R2R3-MYB homolog was obtained from Narcissus pseudonarcissus Pink charm, named NpMYB, which encompassed 333 bp. The 3＇ end of NpMYB was also cloned by 3＇ RACE. Finally, the assembly of NpMYB encom- passed 746 bp, encoding a polypeptide of 202 amino acids. The sequencing analysis showed that amino acid sequence of NpMYB was 77%, 74%, 67%, 68% and 68% homologous with Salvia miltiorrhiza, Petunia x hybrida, Arabidopsis thaliana, Tradescantia fluminensis and Dendrobium so. NpMYB was a typical R2R3-MYB which contained a comvlete R2R3 reveat domain.

关 键 词：洋水仙 MYB 序列分析 

分 类 号：S682.21[农业科学—观赏园艺]