金属硫蛋白对肝脏缺血再灌注大鼠体内酶活性的影响  

THE EFFECT OF METALLOTHIONEIN ON THE ACTIVITY OF SOME ENZYMES IN HEPATIC ISCHEMIC REPERFUSION RAT

作　　者：蒋与刚[1] 郭长江[1] 韦京豫[1] 葛玉章[1] 高兰兴[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《解放军预防医学杂志》2000年第6期396-399,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

摘　　要：目的 :观察金属硫蛋白 (MT)对肝脏缺血再灌注损伤 (HIRI)大鼠体内酶活性的影响 ,旨在为临床应用奠定理论基础。方法 :建立HIRI动物模型 ,并按体重给予不同剂量的MT( 1、2、5mg·kg- 1) ;观察血清转氨酶、抗氧化酶活性及脂质过氧化物含量改变。结果 :与HIRI大鼠比较 ,MT补充组动物血清谷丙转氨酶 (GPT)、谷草转氨酶 (GOT)和乳酸脱氢酶 (LDH)活性明显降低 ;血清和肝组织中丙二醛 (MDA)含量下降 ;全血GSH -Px活性及血清SOD含量升高。三个剂量组中以 1mg·kg- 1MT补充组效果最佳。大鼠血清GPT、GOT、LDH活性分别降为 ( 2 55.50± 12 2 .99)U·L- 1、( 2 0 5.4 0± 76.4 8)U·L- 1、( 2 4 69.50± 90 2 .0 7)U·L- 1,与HIRI组相比 ,P <0 .0 1。结论 :再灌注前适量补充MT可通过抑制HIRI大鼠脂质过氧化、增强抗氧化酶活性 ,从而减轻肝脏缺血性氧化损伤 。Objective:To investigate the influence of MT on the activities of some enzymes so as to provide theoretical bases for clinical use. Methods:Animal model of hepatic ischemic reperfusion injury(HIRI) was set up and the activities of antioxidative enzymes or transaminase,the level of lipid peroxidation were measured after the rats were given MT(1,2 and 5 mg·kg -1 bw,ip).Results:Compared with HIRI rats,the activities of GPT, GOT and LDH in MT supplemented group were significantly decreased,the concentration of MDA in serum and liver were also decreased while the activities of GSH-Px in whole blood and SOD in serum were increased. The 1 mg·kg -1 group was the best among 3 MT supplemented groups.The activities of GPT, GOT and LDH in rat serum decreased to (255.50±122.99) U·L -1 ,(205.40±76.48) U·L -1 and (2 469.50±902.07) U·L -1 respectively.Conclusion:MT is able to reduce the oxidative damage of ischemic liver by inhibiting the lipid peroxidation and elevating the activities of antioxidative enzymes.It has protective effect on hepatic ischemic reperfusion injury.

关 键 词：金属硫蛋白 肝脏缺血再灌注 脂质过氧化 

分 类 号：R657.3[医药卫生—外科学] R151.2[医药卫生—临床医学]