小儿哮喘宁平喘作用的研究  

Research on antiasthmatic effect of Xiao'er Xiaochuanning

作　　者：熊静悦[1] 石莹莹[1] 谭正怀[1] 

机构地区：[1]四川省中医药科学院国家中医药管理局中药质量生物评价重点研究室,四川成都610041

出　　处：《药物评价研究》2013年第5期367-370,共4页Drug Evaluation Research

基　　金：四川省公益性科研院所基本科研项目(A-2011N-8)

摘　　要：目的初步评价小儿哮喘宁的平喘作用,为临床应用提供科学的实验依据。方法运用乙酰胆碱及组胺致豚鼠哮喘模型、内毒素(lipoplysaccharides,LPS)致小鼠急性肺损伤模型、卵白蛋白(ovalbumin,OVA)致豚鼠过敏性哮喘模型及OVA加LPS致小鼠过敏性哮喘模型,评价小儿哮喘宁的平喘作用。结果小儿哮喘宁20、10、5 g/kg均能延长乙酰胆碱+组胺所致豚鼠化学刺激性哮喘的引喘潜伏期及OVA致豚鼠过敏性哮喘的引喘潜伏期并减少跌倒动物数;20 g/kg小儿哮喘宁能降低LPS致小鼠急性肺损伤程度;对嗜酸粒细胞(eosinophil,EOS)浸润型过敏性哮喘动物模型的作用优于对中性粒细胞(neutrophil,NEU)浸润型过敏性哮喘动物模型的作用。结论小儿哮喘宁有平喘的作用,该作用可能与其抑制肺水肿、降低气道EOS浸润有关。Objective To investigate the antiasthmatic effect of Xiao＇er Xiaochuanning and to provide the experimental evidence for its clinical application.Methods The acetylcholine and histamine-induced asthma model in guinea pigs,lipoplysaccharides（LPS）-induced acute lung injury model in mice,ovalbumin（OVA）-induced guinea pig allergic asthma model,and OVA ＋ LPS-induced mice allergic asthma model were used to evaluate the antiasthmatic effect of Xiao＇er Xiaochuanning.Results Xiao＇er Xiaochuanning（20,10,and 5 g/kg） prolonged the latent period in acetylcholine-and histamine-induced asthma of guinea pigs and OVA-induced allergic asthma of guinea pig,and could reduce the number of tumbling animals.Xiao＇er Xiaochuanning（20 g/kg） could reduce the acute lung injury induced by LPS;However,the protective effect against OVA-induced eosinophilic（EOS） inflammation in guinea pigs was better than that of OVA ＋ LPS-induced noneosinophilic（NEU） inflammation in mice.Conclusion The protection of Xiao＇er Xiaochuanning against asthma is related to inhibiting the pulmonary edema and decreasing the eosinophilic infiltration in airway.

关 键 词：小儿哮喘宁 平喘 哮喘 

分 类 号：R985[医药卫生—药品]