小白菊内酯对兔膝骨关节炎血清IL-1β及TNF-α的影响  

Effect of Parthenolide on Serum Expressions of Interleukin-1β and Tumor Necrosis Factor-alpha in Rabbits with Knee Osteoarthritis

作　　者：朱芳晓[1] 周润华[1] 石宇红[1] 覃泱[1] 

机构地区：[1]桂林医学院附属医院风湿免疫科,广西541001

出　　处：《中国中西医结合杂志》2013年第10期1382-1384,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广西壮族自治区中医药民族医药基金资助项目(No.gzzc1239);桂林市科学研究与技术开发计划基金项目(No.2012012-1-9);广西卫生厅科研课题基金资助项目(No.Z2012405)

摘　　要：目的观察小白菊内酯(parthenolide,PTL)对兔膝骨关节炎的治疗作用及其对兔膝骨关节炎血清白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量的影响。方法健康纯种新西兰兔40只,随机选择8只作为正常对照组(简称正常组),其余32只采用右后肢伸直位管型石膏固定法,建立膝骨关节炎模型。造模后随机分为4组:模型对照组(简称模型组)、PTL高剂量组(简称PTL高组)、PTL中剂量组(PTL中组)、PTL低剂量组(PTL低组),每组8只。应用酶联免疫吸附法检测观察各组动物灌服不同剂量的PTL时血清IL-1β和TNF-α的浓度。结果与模型组比较,PTL高、中、低组IL-1β及TNF-α的浓度均下降(P<0.01),且下降的幅度与PTL的浓度呈正相关(IL-1β:r=0.55,P<0.01;TNF-α:r=0.56,P<0.01)。而组间比较,在PTL浓度达到20μmol/L治疗组时抑制达到高峰。结论 PTL能下调兔膝骨关节炎血IL-1β及TNF-α的浓度,且下降的幅度与PTL的浓度呈正相关。Objective To observe the therapeutic effect of parthenolide （PTL） on rabbit knee arthritis （KOA） and its effects on serum expression of interleukin-1β （IL-1β） and contents of tumor necro- sis factor-alpha （TNF-α. Methods Eight rabbits were randomly selected from 40 healthy pure-bred New Zealand rabbits as the normal control group. The KOA model was established in the rest 32 rabbits by plaster cast fixation of the right hind limb extension position. After modeling they were randomly divided into 4 groups, i.e., the model control group, the high dose PTL group, the middle dose PTL group, and the low dose PTL group, 8 in each group. Serum contents of IL-1β and TNF-α were detected using enzyme-linked immunosorbent assay, Results Compared with the model group, IL-1β and TNF-α concentration decreased in the 3 PTL groups （P 〈0.01 ）. The decrement was positively correlated with PTL concentrations （IL-1β： r =0.55, P 〈0.01 ; TNF-α r =0.56, P 〈0.01 ）. The inhibition reached the peak when the PTL concentration arrived at 20 μmol/L. Conclusions PTL could down-regulate the blood IL-1β and TNF-α concentrations of KOA rabbits. Besides, the decrement was positively correlated with the PTL concentration.

关 键 词：小白菊内酯 兔膝骨关节炎 白细胞介素-1Β 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]