活血化瘀、益气补肾中药复方对高糖刺激下大鼠肾小球系膜细胞增殖及纤维连接蛋白表达的影响  

Effect of Chinese Herbal Compounds for Blood Activating Stasis Removing, Qi Benefiting Shen Invigorating on High Glucose Stimulated Proliferation of Renal Mesangial Cells and Expressions of Fibronectin

作　　者：刘丹丹[1] 韩文霞[1] 宋玉文[2] 娄凯[1] 于春晓[1] 高聆[3] 赵家军[1] 

机构地区：[1]山东大学附属省立医院内分泌科,山东省内分泌代谢病临床医学中心,山东省临床医学研究院内分泌与代谢研究所,济南250021 [2]山东省立医院西院麻醉科,济南250022 [3]山东大学附属省立医院中心实验室,济南250021

出　　处：《中国中西医结合杂志》2013年第10期1400-1403,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30701130);国家自然科学基金资助项目(No.81273880)

摘　　要：目的研究活血化瘀、益气补肾中药复方对高糖刺激下大鼠肾小球系膜细胞(RMCs)增殖及纤维连接蛋白(FN)表达的影响。方法以大鼠RMCs为研究对象,用高糖和不同浓度的中药处理RMCs,分别作用24、48 h后,用噻唑蓝(MTT)法检测RMCs的增殖情况,实时定量PCR法检测FN mRNA表达,ELISA法检测FN蛋白表达。结果处理24 h和48 h,与对照组比较,单纯高糖组细胞增殖明显升高(P<0.01,P<0.01);FN mRNA表达及蛋白表达也明显升高(P<0.01);与高糖组比较,24 h时各浓度中药组RMCs增殖及FN蛋白及mRNA表达差异无统计学意义(P>0.05);48 h时各浓度中药组RMCs增殖及FN蛋白及mRNA表达较高糖组明显下降(P<0.01,P<0.01)。结论高糖能够刺激RMCs增殖及FN蛋白及mRNA表达,中药处理24 h对高糖刺激的RMCs增殖及FN蛋白及mRNA表达的作用不明显,中药处理48 h能够逆转高糖刺激下RMCs的增殖及降低FN蛋白及mRNA的表达。Objective To study effects of Chinese Herbal Compounds （CHC） for blood activating stasis removing （BASR）, qi benefiting Shen invigorating （QBSI） on high glucose stimulated proliferation of renal mesangial cells （RMCs） and expressions of fibronectin （FN）. Methods Rats＇ RMCs were dealt with high glucose and different concentrations of Chinese medicine for 24 and 48 h respectively. The proliferation of RMCs was detected with 4-A thiazolyl blue. mRNA expressions of FN was detected by real time quantitative PCR. The protein expression of FN was detected by ELISA. Results Compared with the control group, the proliferation obviously increased （P 〈0.05, P 〈0.01 ） after 24 and 48 h of treatment in the high glucose group, mRNA and protein expressions of FN also increased （P 〈0.01 ）. There was no statistical difference in the proliferation of RMCs or expressions of FN at 24 h between each CHC group and the high glucose group （P 〉0.05）. Compared with the high glucose group, the proliferation of RMCs and expressions of FN at 24 h each obviously decreased in the CHC group （P 〈0.05, P 〈0.01 ）. Conclusions High glucose could promote the proliferation of RMCs and induce expressions of FN. No obvious effect could be stimulated by CHC treatment for 24 h. The proliferation of RMCs, protein and mRNA expressions of FN could be reversed by CHC treatment for 48 h.

关 键 词：中药复方 肾小球系膜细胞 纤维连接蛋白 

分 类 号：R285.5[医药卫生—中药学]