妊娠高血压综合征大鼠血清硫化氢浓度的变化及意义  

Change and Significance of Serum Hydrogen Sulfide Levels in Rats with Pregnancy-induced Hypertension Syndrome

作　　者：周建斌[1] 刘小叶[1] 欧阳新平[2] 何平平[3] 王波[4] 周寿红[2] 潘思[4] 唐艳艳[2] 李元辉[2] 李额 

机构地区：[1]南华大学附属第二医院妇产科,湖南省衡阳市421001 [2]南华大学医学院生理学教研室心血管疾病研究所,湖南省衡阳市421001 [3]南华大学护理学院,湖南省衡阳市421001 [4]南华大学附属第一医院,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2013年第9期797-801,共5页Chinese Journal of Arteriosclerosis

基　　金：2012年湖南省高校科研项目(12C0350);湖南省科学技术发展计划资助项目(2013FJ3027);衡阳市科技局项目资助(2012KJ22)

摘　　要：目的探讨妊娠高血压综合征大鼠血清硫化氢(H_2S)的浓度变化及其意义。方法成年雌性Wistar大鼠32只,随机分为正常对照组、正常妊娠蛆、妊娠高血压综合征组和妊娠高血压综合征+硫氢化钠组,采用腹腔注射一氧化氮合酶抑制剂L-NAME[250 mg/(kg·d)]建立妊娠高血压综合征大鼠模型。采用套尾法测量大鼠收缩压,分光光度法测量血清H_2S浓度,自动生化分析仪测定尿蛋白、血肌酐和血尿素氮。测量胎鼠身长及胎鼠、胎盘和胎头重量。结果与正常妊娠组比较,妊娠高血压综合征组大鼠收缩压、尿蛋白、血肌酐和血尿素氮水平显著增加(P<0.05);胎鼠宫内发育迟缓,胎鼠重量、胎盘重量、胎头重量和胎鼠身长显著减小(P<0.05);血清H_2S水平从妊娠第10天开始显著降低(P<0.05),直到分娩实验结束。外源性H_2S逆转了上述改变。结论妊娠高血压的发生可能与内源性H_2S的减少有关,外源性H_2S可抑制妊娠高血压综合征的发生。Aim To investigate the change and significance of serum hydrogen sulfide levels in rats with preg- nancy-induced hypertension syndrome （PIHS）. Methods Thirty two adult female Wistar rats were randomly divided into normal control group, normal pregnant group, PIHS group and PIHS ＋ sodium hydrosulfide （NariS） group. The pregnant rats were treated with nitric oxide synthase inhibitor L-nitro-arginine methylester （L-NAME） [ 250 mg/（ kg ~ d） ] by intraperitoneal injection to induce hypertension. The extrogenous sodium hydrosulfide （H^S） donator NariS （56 ~mol/kg） was used in the experiment. Systolic blood pressure （SBP） of rat was measured by use of tail-cuff. The level of H2 S in serum was detected by spectrophotometry. The levels of urine protein, blood creatinine （BCr） and blood urea nitrogen （BUN） were measured by automatic biochemistry analyzer. The length and weight of fetal rat, weight of placenta and head of fetal rat were measured. Results Compared with the normal pregnant group, SBP, urine protein, BCr and BUN were singificantly increased in PIHS group. The fetal rats in the uterus were hypoevolutism and the length and weight of fetal rat, weight of placenta and head of fetal rat were singificantly decreased in PIHS group compared with the normal pregnant group. The levels of H2S in serum were singificantly decreased from day tenth of pregnancy to start in PIHS group compared with the normal pregnant group. Conclusion The decrease of endogenous H2 S may be anim- portment reason for the PIHS and extrogenous H2S inhibits the formation of PIHS in rats.

关 键 词：妊娠高血压综合征 硫化氢 亚硝基左旋精氨酸甲酯 大鼠 

分 类 号：R714[医药卫生—妇产科学]