冠心病患者血浆胆固醇酯转运蛋白水平与高密度脂蛋白亚类组成的关系  

Relationship Between Levels of CETP and Distribution of Plasma HDL Subclasses in Patients with Coronary Heart Disease

作　　者：丁岚[1] 田英[1] 乔新惠[1] 周永强[1] 陈浩[2] 王小鸥[2] 刘英[1] 龙石银[1] 

机构地区：[1]南华大学生物化学与分子生物学教研室,湖南省衡阳市421001 [2]南华大学附属第一医院,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2013年第9期812-816,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30800474)资助;湖南省科技厅项目(2010SK3037);湖南省教育厅重点项目(11A104);湖南省研究生创新项目(CX2010B385;CX2011B378)资助;湖南省重点学科建设项目;南华大学"十二五"科技创新团队项目资助

摘　　要：目的探讨冠心病患者胆固醇酯转运蛋白(CETP)水平与血浆高密度脂蛋白(HDL)亚类组成的关系。方法收集冠心病患者血浆116例和健康对照组血浆87例,采用双向电泳-免疫印迹检测法分析其HDL亚类浓度,用酶联免疫法测定CETP浓度。按CETP浓度均值加或减去一个标准差作为分割点,将冠心病患者分为3组:低CETP组(CETP≤0.69 mg/L)、中CETP组(0.69<CETP<1.59 mg/L)、高CETP组(CETP≥1.59 mg/L)。结果随着CETP浓度的升高,冠心病患者血浆总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平呈增加趋势,高密度脂蛋白胆固醇、载脂蛋白A1水平和载脂蛋白A1/B100比值呈减少趋势。与低CETP组相比,中CETP组和高CETP组preβ_1-HDL及HDL_(3c)含量增加,而HDL_(2b)含量降低(P<0.05或P<0.001)。直线相关和多元回归分析中发现,血浆CETP水平与preβ_1-HDL浓度呈正相关,与HDL_(2b)浓度呈负相关。结论随着血浆CETP水平的升高HDL颗粒有变小的趋势,CETP可能阻碍了HDL的成熟代谢。Aim To study the relationship between the levels of cholesteryl ester transfer protein （CETP） and the contents of plasma high density lipoprotein （HDL） subclasses in patients with coronary heart disease （CHD）. Methods The contents of plasma HDL subclasses in healthy controls （ n -- 116） and the CHD subjects （ n = 87 ） were de- termined by two-dimensional gel electrophoresis associated with immunodetection method. The levels of CETP were deter- mined by enzyme-linked immunosorbent assay （ELISA） kits. According to the plasma CETP levels, the CHD subjects were divided into three layers amount [ low CETP group （ CETP〈0. 69 mg/L）, middle CETP group （0. 69 〈 CETP 〈 1.59 mg/L）, high CETP group （CETP〉1. 59 mg/L） ]. Results With the increase of CETP concentration, the contents of total cholesterol, triglyceride, low density lipoprotein cholesterol were increased significantly, while that of high density lipoprotein cholesterol, ApoA1 and ApoA1/B100 were decreased significantly. Compared with the low CETP group, pre[5^-HDL and HDLac were significantly increased, but HDL2b was significantly decreased in middle CETP group and high CETP group （P 〈 0. 05 or P 〈 0. 001 ）. The plasma CETP was positively correlated with the contents of prel31 -HDL and negatively correlated with the contents of HDL2b. Conclusions With elevated plasma CETP levels, the particle size of HDL in CHD subjects showed a shift toward smaller size, CETP may hinder mature metabolism of HDL.

关 键 词：高密度脂蛋白亚类 胆固醇酯转运蛋白 动脉粥样硬化 冠心病 

分 类 号：R5[医药卫生—内科学]