γ射线照射与吸入苯、甲苯加CO的复合效应研究  

Research on biological effects induced by γ-irradiation combined with benzene,toluene and carbon monoxide inhalation.\

作　　者：王月兴[1] 张鸿源[1] 王兰金[1] 潘燕[1] 范正平[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《中华放射医学与防护杂志》2000年第5期301-305,共5页Chinese Journal of Radiological Medicine and Protection

基　　金：国防科工委重点资助!项目 (9.2 .6② )

摘　　要：目的　探索γ射线、苯、甲苯和CO同时作用时的生物效应规律 ,并分析各因素之间的交互作用性质 (拮抗、相加或协同 )。方法　用家兔 90只分成 9组 ,其中一组为对照组 ,按L8(2 7)正交表设计进行 4因素二水平的 8组实验。各因素的两种暴露水平分别是 :γ射线为 0 0 0 75和0 0 375Gy/d ;苯为 40± 15和 (182± 33)mg/m3;甲苯为 90± 30和 (4 0 7± 6 8)mg/m3;CO为 (93± 4)和 (2 78± 8)mg/m3。暴露实验在密闭舱内进行 ,每天 2h ,每周 5d ,持续 8周。对实验结果用方差分析和组间比较进行了处理。结果　 (1)γ射线、苯和甲苯都能诱发外周血淋巴细胞染色体畸变、微核、姐妹染色单体互换 (SCE)以及骨髓细胞染色体畸变 ,但CO对上述各项指标均无明显单独作用。(2 ) 4个因素的综合作用与 4个因素单独作用产生的效应总和的比值ω对淋巴细胞的双着丝点加环、无着丝粒、畸变细胞、总畸变、微核及SCEs/C分别为 2 16、1 5 8、2 0 7、2 6 7、1 2 5和 1 18,对畸形精子率则高达 5 97。各因素之间呈明显的协同作用。但苯和γ射线对淋巴细胞无着丝粒有明显拮抗作用。 (3) 4个因素都引起睾丸重量指数降低 ;γ射线、甲苯和CO都能引起精子数减少和畸形精子率增高 ;结论　γ射线、苯和甲苯对诱发某些生物效应具有很显著的?Objective\ To explore the patterns of bilogical effects induced by γ-rays irradiation combined with slmultaneous inhalation benzene,toluene or carbon monoxide and to analyze their antagonistic,additive or synergistic interaction. Methods\ Ninety healthy male rabbits were equally divided into 9 groups.Eight of which were asigned to be test groups according to four-factor-two-dose-level orthogonal layout ｛L 8(2 7)｝ program and the other one was the control group.The two dose-levels of these four agents were:the γ-irradiation doses were 0 0075 Gy/d and 0 0375Gy/d,and the two concentrations of benzene,toluene and carbon monoxide were 40±15 and 162±33 mg/m 3,90±30 and 407±68 mg/m 3,93±4 and 278±8 mg/m 3,respectively.The animals were exposed to γ-irradiation combined with benzene,toluene or CO vapour 2 h a day and 5 days a week for successive 8 weeks.Variance analysis and comparison between test groups were made for analyzing the test data. Results\ (1)It was showed that γ-irradiation,benzene and toluene could all induce chromosome aberrations,SCEs and micronuclei of lymphocytes and chromosome aberrations of bone marrow cells;but no effect could be seen in CO alone treated group.(2)The ratios (ω) of biological effects jointly induced by the four agents and the sum of those induced separately by them were 2 16,1 58,2 07,2 67,1 25 and 1 18 for dicentric+ring,acentric,aberration cells,total aberration,micronuclei and micronucleus cells,respectively,and it was as high as 5 97 for aberrant sperms.The ratios showed that the interactions were synergistic(ω>1).However,interactions between γ-rays and benzene was antagonistic for acentric of lymphocytes.(3)The four agents could all obviously cause decrease of weight index of testis,γ-rays,toluene and CO could all markedly reduce the number of sperms and increase the ratio of aberrant sperms. Conclusion γ-irradiation combined with benzene,toluene and CO inhalation can lead to significant interactions which are mainly synergistic,while CO can caus

关 键 词：Γ射线 苯 甲苯 生物效应 交互作用 一氧化氮 

分 类 号：R14[医药卫生—公共卫生与预防医学]