番木瓜主栽品种SCoT指纹图谱构建及遗传变异分析  

SCoT Fingerprints and Genetic Variations of the Papaya (Carica papayaL.)Major Cultivars

作　　者：杨祥燕 蔡元保 黄秋伟 陈涛 覃剑峰 彭靖茹 

机构地区：[1]广西亚热带作物研究所,南宁530001

出　　处：《西北植物学报》2013年第9期1756-1761,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：广西直属公益性科研院所基本科研业务费专项(桂热研201006);国家公益性行业(农业)科研专项(201203021)

摘　　要：利用SCoT标记对中国22个番木瓜主要栽培品种进行遗传多样性分析和指纹图谱构建,为番木瓜品种资源的鉴定和杂交亲本的选配提供技术参考。结果显示：（1）SCoT标记有效等位基因数（Ne）平均为1.47,Nei’s基因多样性指数（H）平均为0.26,Shannon’s信息指数（I）平均为0.39,表明SCoT标记具有较高的多态性检测效率。（2）聚类分析和主坐标分析结果表明,大部分番木瓜品种间的遗传相似系数为0.79~0.90,品种间遗传多样性水平较低;在相似性系数为0.75时可将所有品种分为3个组群。（3）6个SCoT引物可以将22个番木瓜品种完全区分开,每个品种都各有独特的指纹图谱,置信概率达到99.976%。The aim of this study was to construct a DNA fingerprinting database of 22 papaya major cultivars in China,and the genetic diversity was analyzed based on start condon targeted polymorphism（SCoT）.The results are：（1）Effective number of alleles（Ne）,Nei＇s gene diversity（H）and Shannon＇s information index（I）of SCoT were 1.47,0.26 and 0.39,respectively.The results indicated that SCoT markers could detect higher genetic diversity in the papaya cultivars.（2）UPGMA cluster analysis and principal coordinates analysis（PCOA）showed that the range of genetic similarity（GS）was 0.79~0.90 in most of the papaya cultivars with a low genetic diversity.All cultivars could be divided into three groups at the genetic similarity of 0.75.（3）The 22 cultivars of papaya could be absolutely identified by six SCoT primers with 99.976% probability of confidence and every cultivar of papaya had a unique fingerprint map.These results provide a beneficial reference for genetic breeding of papaya in China.

关 键 词：番木瓜 主栽品种 SCoT 指纹图谱 遗传多样性 

分 类 号：Q789[生物学—分子生物学]