腐植酸对高温胁迫下掌叶半夏生长生理特性及块茎次生代谢的影响  

Effect of Humic Acid on Tubers Secondary Metabolism and Growth Physiological Property of Pinellia pedatisecta under High Temperature Stress

作　　者：王乾[1] 王康才[1] 崔志伟[1] 吴晓燕[1] 

机构地区：[1]南京农业大学中药材研究所,南京210095

出　　处：《西北植物学报》2013年第9期1845-1850,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：南京农业大学校级SRT计划项目

摘　　要：采用不同浓度(0、20、40、60、80、100mg/L)腐植酸(HA)叶面喷施处理,通过盆栽实验分析了HA对高温胁迫下掌叶半夏幼苗生长生理指标及次生代谢的调控效应。结果显示:(1)各浓度HA处理均可不同程度地促进高温胁迫下掌叶半夏的生长发育,且以80mg/L HA处理下其块茎鲜重(6.990 5g/株)、叶柄鲜重(1.755 4g/株)及总叶绿素含量(19.961 3mg/g)最高,较对照分别显著提高21.25%、118.5%和37.19%。(2)当HA处理浓度为60mg/L时,掌叶半夏幼苗叶片中可溶性糖、可溶性蛋白含量均最高,分别比对照显著提高66.67%、40.91%;叶片抗氧化酶(SOD、POD)活性达到最大值,MDA含量降至最低,SOD、POD活性分别比对照显著提高818.98%和48.2%,MDA含量较对照显著降低62.08%;且块茎中鸟苷含量较高,比对照显著提高52.94%。(3)当叶面喷施80mg/L HA时,掌叶半夏幼苗叶片中游离脯氨酸含量和块茎中总生物碱、总有机酸、腺苷的含量均最高,分别比对照显著提高169.63%、27.19%、42.32%、96.23%。研究表明,适宜浓度HA处理可显著提高高温胁迫下掌叶半夏幼苗叶片内抗氧化酶(SOD、POD)的活性及渗透调节物质(可溶性糖、可溶性蛋白、游离脯氨酸)含量,以及块茎中次生代谢产物(总生物碱、总有机酸、鸟苷和腺苷)的含量,有效减轻夏季高温对掌叶半夏幼苗叶片的伤害、延缓衰老、提高其幼苗抗热性,促进生长、延长生长期。Humic acid is a kind of structure,material of a similar nature of complex compound.At the same time,it is a kind of hydrophilic colloid,and with a variety of groups.We conducted an experiment to investigate the effect of HA on physiological indexs and secondary metabolism.We set up five different leaf spray application concentration gradient（0,20,40,60,80,100 mg/L）of HA.Using potted trial,we analyzed the influence of the physiological indexes by HA on Pinellia pedatisecta seedlings under high temperature stress,as well as the regulation effect of secondary metabolism.The results showed that：（1）Each HA concentration treatment can promoted the growth of P.pedatisectain different degrees under high temperature stress.With the treated of 80 mg/L,the fresh weight of tubers,petiole and the total chlorophyll content is the highest,compared with the control has significantly increased 21.25%,118.5% and 37.19%,respectively.（2）Compared with the control,under the concentration of 60 mg/L,the content of soluble sugar,soluble protein in leaves were highest.Both respectively increased by 66.67% and 40.91%,respectively.The activities of antioxidant enzymes（SOD,POD）were the highest,with significantly increased by 818.98% and 48.2%,respectively.The content of MDA decreased significantly by 62.08%,and the content of guanosine in tuber was significantly increased by 52.94%.（3）Under 80 mg/L,the content of free proline in leaves and the content of total alkaloid,total organic acid and adenosine of tubers are the highest.And compared with the control they are significantly improved by 169.63%,27.19%,42.32% and 96.23%,respectively.Researches showed that fitting consistency of HA treatment could significantly increase the activities of antioxidant enzymes（SOD,POD）and the content of osmoregulation substance（soluble sugar,soluble protein and free proline）.At the same time improved the metabolism of active ingredients contents accumulation（total alkaloid,total organic acid,adenosine and guanosine）in the tu

关 键 词：掌叶半夏 腐植酸 高温胁迫 次生代谢 生理特性 

分 类 号：Q945.78[生物学—植物学]