薄壳山核桃花粉离体萌发和花粉管生长特性研究  

in vitro Pollen Germination and Tube Growth Characteristics in Pecan(Carya illinoinensis)

作　　者：张瑞[1] 李洋[1] 梁有旺[1] 彭方仁[1] 李永荣 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]南京绿宙薄壳山核桃科技有限公司,南京210014

出　　处：《西北植物学报》2013年第9期1916-1922,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：林业公益性行业科研专项(201304711);江苏省农业科技支撑项目(BE2010311);国家科技推广项目[2011(33)]

摘　　要：以6年生薄壳山核桃优良品种‘金华’的新鲜花粉为试材,采用离体培养法,研究了不同复水时间、不同培养基组分、不同培养温度及培养时间对薄壳山核桃花粉萌发和花粉管生长的影响。结果显示：（1）薄壳山核桃花粉萌发试验前进行4h复水处理可显著提高萌发率,萌发率可达51.78%,为对照（2.51%）的20.68倍。（2）蔗糖、H3BO3、Ca（NO3）2·4H2O在一定浓度范围内均具有促进花粉萌发和花粉管生长的作用,但浓度过高则起抑制作用。（3）正交试验结果经实验验证表明,薄壳山核桃花粉萌发和花粉管生长的最适培养基为20%蔗糖＋0.02%~0.03%H3BO3＋0.05%Ca（NO3）2·4H2O,最佳培养条件为25℃下恒温培养24h,此时的花粉萌发率高达74.46%,花粉管平均长度为258.84μm。The fresh pollens of 6 a ‘Jinhua’pecan（Carya illinoinensis）tree were used in this study.The focus was to investigate the regulatory role of sucrose,boric acid,Ca2＋,rehydration time and temperature in pecan pollen germination and pollen tube growth by in vitro culture.And the results showed that：（1）The rehydration for 4 h before the test is necessary which could improve pollen germination significantly;（2） Sucrose,boric acid and Ca2＋ could stimulate pollen germination and tube growth,but this function would reverse if overrun certain concentration;（3）The research suggested that the optimum culture medium consist of 20% sucrose,0.02%~0.03% H3BO3 and 0.05% Ca（NO3） 2 ·4H2O and the pollen germination percentage was nearly 74.46% and the average pollen tube length reached 258.84 μm after a 24 h incubation at 25℃.

关 键 词：薄壳山核桃 培养基 花粉萌发 花粉管生长 培养条件 

分 类 号：Q813.1[生物学—生物工程]