μ阿片受体基因内含子2多态性与美沙酮维持治疗剂量的关联分析  

Association analysis of polymorphisms in intron 2 of μ opioid receptor gene and effective dose in methadone maintenance treatment of heroin addiction

作　　者：谢小虎[1,2] 刘惠芬[1,2] 张亚海[1,2] 陈为升[1] 庄丁丁[1] 张建兵[3,4] 周文华[1,2,3,4] 

机构地区：[1]宁波市微循环与莨菪类药研究所 [2]宁波大学行为神经科学研究中心 [3]宁波戒毒研究中心 [4]浙江省戒毒治疗研究中心

出　　处：《中国临床药理学与治疗学》2013年第10期1081-1085,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(81071077);宁波市科技项目(2007A22008;2013A610252)

摘　　要：目的:探讨μ阿片受体基因内含子2的两个单核苷酸多态性位点(Single nucleotide polymorphism,SNP)rs9479757和rs2075572与海洛因成瘾者美沙酮维持治疗剂量的相关性。方法:应用TaqMan荧光技术检测68例美沙酮维持治疗病例的rs9479757和rs2075572的基因型并进行与美沙酮维持剂量高低的关联分析。结果:rs9479757和rs2075572在美沙酮维持治疗高、低剂量组间的分布差异无统计学意义;rs9479757和rs2075572各基因型与美沙酮维持治疗剂量差异无统计学意义。结论:μ阿片受体基因内含子2的两个SNP(rs9479757和rs2075572)多态性与海洛因成瘾者美沙酮维持剂量的高低无明显相关性。AIM： To explore the association between the single nucleotide polymorphisms （SNP） in intron 2 of the opioid receptor gene （OPRM1） and the effective dose in methadonemaintenance METHODS ： （rs9479757, patients in treatment of heroin addiction. The genotypes of the two SNPs rs2075572） in 68 heroin dependent methadone maintenance treatmentwere detected by TaqMan SNP genotyping tech nique, and the association between methadone dose and the two SNPs was analyzed. RE SULTS.The frequencies of genotype and allele at rs9479757, rs2075572 were not significantly dif ferent between the high and low methadone dose groups （P〈0.05）. The average methadone dai ly dosages of various genotypes at the two SNPs were not significantly different （P 〈 0.05）.CONCLUSION ： The polymorphisms （rs9479757, rs2075572） in intron 2 of the opioid receptor gene （OPRM1） may not play the major role in the effective dose in methadone maintenance treatment of heroin addiction.

关 键 词：Μ阿片受体 基因多态性 海洛因 美沙酮 

分 类 号：R969.8[医药卫生—药理学]