洗心汤对SAD大鼠脑内tau蛋白O-GlcNAc糖基化修饰相关酶的影响  

Effects of Xixin decoction on enzymes related to O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease

作　　者：第五永长[1] 田金洲[2] 时晶[2] 

机构地区：[1]陕西中医学院中医临床医学院,陕西咸阳712046 [2]北京中医药大学第一临床医学院,北京100029

出　　处：《南方医科大学学报》2013年第10期1442-1447,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(30973738)~~

摘　　要：目的建立SAD动物模型,研究名方洗心汤对SAD模型大鼠脑组织tau蛋白O-GlcNAc糖基化修饰中蛋白质O位N-乙酰葡萄糖胺糖基转移酶(OGT)、和糖苷酶(O-GlcNAcase)的影响,探讨名方洗心汤防治SAD的可能作用机制。方法将SPF级健康雄性SD大鼠随机分为假手术组、模型组、多奈哌齐对照组、名方洗心汤小、中、大剂量组。治疗结束及行为学测试之后,以免疫组化及蛋白印迹方法检测大鼠脑组织tau蛋白O-GlcNAc糖基化修饰过程中OGT、O-GlcNAcase酶表达的变化。结果名方洗心汤能显著提高SAD大鼠海马OGT的表达,降低O-GlcNAcase的表达,组间差异有统计学意义(P<0.05,P<0.01)。多奈哌齐对照组与SAD模型组相比无显著性差异(P>0.05)。结论名方洗心汤能够调节O-GlcNAc糖基转移酶和糖苷酶的表达,从而提高SAD大鼠海马组织tau蛋白O-GlcNAc糖基化修饰水平。Objective： To study the effects of Xixin decoction （XXD） on O-GIcNAc transferase （OGT） and O-GlcNAc glycosidase in O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer＇s disease （SAD） and explore the possible mechanism. Methods Male SD rats were randomly divided into sham-operated group, model group, donepezil group, and low-, moderate-, and high-dose XXD groups. After treatment and behavioral test, the rats were sacrificed for detecting the expressions of OGT and O-GlcNAc glycosidase in the brain using immunohistochemistry and Western blotting. Results XXD significantly enhanced the expressions of OGT in the hippocampus of SAD rats and lowered the expression of O-GlcNAc glycosidase （P〈0.05 or 0.01）. OGT and O-GlcNAc glycosidase expressions showed no significant differences between the model group and donepezil group （P〉0.05）. Conclusion XXD can regulate the expression of OGT and O-GlcNAc glycosidase to enhance O-GIcNAc glycosylation of tau proteins in the hippocampus of SAD rats.

关 键 词：散发性老年性痴呆 洗心汤 SAD模型 O—GlcNAc糖基转移酶 O-GlcNAc糖苷酶 

分 类 号：R285.5[医药卫生—中药学]