小白菊内酯对高糖诱导的大鼠肾小球系膜细胞增殖及核因子-κB、单核细胞趋化蛋白-1表达的影响  

Effects of parthenolide on high glucose-induced cell proliferation, NF-κB activation and MCP-1 expression in rat mesangial cells

作　　者：许兆忠[1] 贾倩倩[2] 李红瑜[2] 王建成[2] 赵艳[2] 陈斯佳[2] 龙海波[2] 

机构地区：[1]南方医科大学珠江医院急诊科,广东广州510282 [2]南方医科大学珠江医院肾内科,广东广州510282

出　　处：《南方医科大学学报》2013年第10期1471-1473,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(81072848);国家级大学生创新创业训练计划项目(201212121021);广东省科技计划项目(2011B061300014)~~

摘　　要：目的探讨小白菊内酯(PTL)对高糖诱导的大鼠肾小球系膜细胞(MC)增殖、核因子-κB(NF-κB)活性和单核细胞趋化蛋白-1(MCP-1)表达的影响。方法采用正常葡萄糖组(糖浓度为5.6 mmol/L)和高糖组(糖浓度为30 mmol/L)、高糖+PTL组,分别体外培养大鼠肾小球MC,MTT法检测MC的增殖,ELISA法检测培养液上清中MCP-1的浓度,Western Blot法检测IκBα反映NF-κB的活性,EMSA法检测NF-κB的活性。结果高糖诱导后MC增殖、MCP-1表达和NF-κB活性明显增强,NF-κB结合蛋白IκBα明显降解;应用PTL干预后,MC增殖、MCP-1表达和NF-κB活性均明显降低,IκBα降解被抑制。结论 PTL能抑制高糖诱导的肾小球MC NF-κB活化及MCP-1的表达,从而为PTL用于临床防治糖尿病肾病提供了一定的理论依据。Objective To explore the effects of parthenolide （PTL） on high glucose-stimulated cell proliferation, NF-JcB activation and monocyte chemoattractant protein-1 （MCP-1） expression in rat mesangial cells （MCs）. Methods MCs were cultured in normal glucose （5.6 mmol/L）, high glucose （30 mmol/L）, or high glucose with PTL. MTT assay was used to detect the cell proliferation. MCP-1 content in the supernatant was measured by ELISA, and the level of IkBa was detected by Western blotting to reflect NF-κB activity. EMSA method was used to measure the activation of NF-κB. Results MC proliferation, MCP-1 expression and NF-κB activation were significantly enhanced and the expression of NF-κB-binding protein IκBa was obviously reduced in cells cultured in high glucose. Application of PTL obviously abolished the effects of high glucose. Conclusion PTL can suppress high glucose-stimulated NF-κB activation and MCP-1 expression in rat MC. These data provide a theoretical basis for the clinical application of PTL in prevention and control of diabetic nephropathy.

关 键 词：小白菊内酯 糖尿病肾病 核因子-ΚB 单核细胞趋化蛋白-1 

分 类 号：R285.5[医药卫生—中药学]