蝴蝶兰抗冷性株系的鉴定与筛选  

Identifying and screening cold tolerance genetypes of Phalaenopsis varieties

作　　者：李杰[1] 匡萍[1] 王芝娜[1] 张银洁[1] 

机构地区：[1]西南科技大学生命科学与工程学院

出　　处：《北京林业大学学报》2013年第5期91-96,共6页Journal of Beijing Forestry University

基　　金：四川省教育厅重点项目(10zd1129);四川省科学技术带头人培养项目(11sd3105);西南科技大学博士基金项目(09zx7107)

摘　　要：为了获得抗冷性蝴蝶兰株系,测定了低温胁迫及温度回升对Phalaenopsis‘Taida Salu’×P.‘Dinghan Orange Anne’杂交后代4个不同株系及亲本的抗氧化酶活性、渗透调节物质和膜脂过氧化作用的影响,用主成分分析对各指标的变化率进行综合评价分析。结果表明:抗冷性程度不同的株系超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性所表现出来的差异,随温度降低,差值呈增大趋势;抗冷性强的株系在温度回升到正常条件时,可溶性糖、脯氨酸和丙二醛(MDA)含量、电解质渗漏率能恢复至处理前水平,而抗冷性弱的株系难以恢复。综合评价排序表明,P6为强抗冷株系,P4、P3为中抗冷株系,其他为弱抗冷株系。Four different offspring varieties of Phalaenopsis ＇ Taida Salu＇ × P. ＇ Dinghan Orange Anne＇ and their parentals were selected to compare their tolerance to coldness. Their antioxidase activities, osmoregulation substance and membrane lipid peroxidation were detected under low temperature stress and recovery. And their tolerance to coldness was evaluated comprehensively using principal component analysis. The results showed that the differences of SOD, POD and CAT activities among varieties were significant. The contents of soluble sugar, free proline and MAD, electrolytic leakage in stronger cold resistance varieties could be equal and even higher than that before treatment, but the cold sensitive varieties were the contrary in the temperature recovery period. The final scores got from principal component analysis showed that P6 had strong tolerance to coldness, P4 and P3 had moderate and others had weak tolerance to coldness.

关 键 词：蝴蝶兰 抗冷性 生理指标 

分 类 号：S682.31[农业科学—观赏园艺]