原发性高血压患者动脉血管组织E26转录因子1及单核细胞趋化蛋白1的表达和临床意义  

Expression and clinical implications of E26 transformation-specific-1 and monocyte chemoattractant protein-1 in arterial tissues of essential hypertensives

作　　者：李从圣[1] 孟令毅[1] 徐俊[1] 程立顺[1] 杨静[1] 程元光 吴伟 

机构地区：[1]安徽医科大学第三附属医院(合肥市第一人民医院)急诊部,安徽合肥230061 [2]安徽医科大学第三附属医院(合肥市第一人民医院)胃肠外科,安徽合肥230061 [3]安徽医科大学第三附属医院(合肥市第一人民医院)胸心外科,安徽合肥230061

出　　处：《中华高血压杂志》2013年第9期855-860,共6页Chinese Journal of Hypertension

基　　金：合肥市科研计划资助项目(201015);安徽医科大学校基金资助项目(2011xkj084)

摘　　要：目的观察E26转录因子1(Ets-1)及其下游靶基因的表达产物单核细胞趋化蛋白1(MCP-1)在原发性高血压患者动脉血管组织中的表达,探讨其在原发性高血压患者血管重构中的作用。方法收集2010-07-12安徽医科大学第三附属医院(合肥市第一人民医院)原发性高血压患者(高血压组,n=17)及正常血压者(正常血压组,n=16)手术废弃组织中的动脉血管,采用免疫组织化学法结合图像信号分析技术及逆转录聚合酶链反应(RT-PCR)方法,检测动脉血管组织中Ets-1、MCP-1蛋白及mRNA的表达。结果高血压组动脉血管组织中Ets-1[肠系膜动脉(186.4±2.4)比(102.8±1.9);肋间动脉(153.4±2.0)比(101.2±1.6)]、MCP-1[肠系膜动脉(115.3±1.0)比(83.2±0.7);肋间动脉(122.4±0.8)比(70.1±0.6)]蛋白及mRNA的表达明显高于正常血压组(均P<0.05);Pearson相关分析显示,高血压组动脉血管组织Ets-1与MCP-1mRNA表达呈正相关(肠系膜动脉,r=0.87;肋间动脉,r=0.83,均P<0.01)。结论与正常血压者相比,Ets-1和MCP-1在原发性高血压患者血管组织中的表达明显上调,两者的表达呈正相关。提示Ets-1、MCP-1在原发性高血压血管重构可能起重要作用。Objective To investigate the expression of E26 transformation-specific-1 （Ets-1） and its downstream target gene expression product monocyte chemoattractant protein-1 （MCP-1） in arterial tissues, and to explore their roles in vascular remolding of essential hypertensive patients. Methods From July 2010 to December 2010, sam ples of arteries from hypertensive patients （ hypertensive group, n = 17） and normotensive controls （normotensive group, n= 16） after surgeries were collected in Third Affiliated Hospital of Anhui Medical University （First People＇s Hospital of Hefei）. The protein and mRNA levels of Ets-1 and MCP-1 in artery tissues were detected by immuno histochemical method and reverse transcription-polymerase chain reaction （RT-PCR）. Results As compared with the normotensive group, the protein expressions of Ets-1 and MCP-1 in hypertensive mesenteric arteries [（ 186.4± 2.4） vs （102.8±1.9）; （115.3±1.0） vs （83.2±0.7）] andintercostals arteries [（153.4±2.01 vs （101.2±1.6）; （122.4±0.8） vs （70.1±0.6）, all P〈0.05] increased markedly. So did the mRNA levels of Ets-1 and MCP-1. A positive correlation between Ets-1 and MCP-1 in arterial tissues of hypertensive patients was found by Pearson correlation analysis with r = 0.87 in mesenteric arteries and r = 0.83 in intercostal arteries （all P 〈 0.01）. Conclusions Compared with the normotensive group, the expressions of Ets-1 and MCP-1 are significantly up-regulated in vessel tissues of hypertensive patients. Furthermore, the expression of Ets-1 and MCP-1 is positively correlated. Which implicates that Ets-1, MCP-1 may play an important role in vascular remolding of essential hypertension.

关 键 词：高血压 E26转录因子1 单核细胞趋化蛋白1 

分 类 号：R544.1[医药卫生—心血管疾病]