沙葱多糖对绵羊外周血淋巴细胞信号转导相关分子的影响  

Effects of Allium Mongolicum Regel polysaccharides on signal molecules in peripheral blood lymphocytes of sheep

作　　者：赵飞艳[1] 敖长金[1] 萨茹丽[1] 包美艳[1] 

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018

出　　处：《细胞与分子免疫学杂志》2013年第11期1125-1128,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30760173)

摘　　要：目的研究体外条件下不同浓度沙葱多糖对绵羊外周血淋巴细胞信号分子生成的影响。方法用(0、50、100、150、200、250)μg/mL等6个沙葱多糖浓度梯度,在体外培养条件下刺激绵羊外周血淋巴细胞,采用Fluo-3/AM荧光探针法测定淋巴细胞培养液中Ca2+浓度、硝酸还原法测定淋巴细胞培养液中一氧化氮(NO)含量;125I同位素标记竞争蛋白结合放射免疫法测定沙葱多糖作用3、6、9 h时细胞内cAMP和cGMP含量。结果沙葱多糖高浓度剂量组可显著提高绵羊外周血淋巴细胞Ca2+浓度(P<0.05),促进外周血淋巴细胞生成NO;沙葱多糖持续作用3 h,随着沙葱多糖浓度的升高,cAMP与cGMP水平呈下降趋势;持续作用6 h与9 h,250μg/mL沙葱多糖组cAMP与cGMP水平显著高于对照组(P<0.05),但其变化无规律。结论沙葱多糖可提高外周血淋巴细胞Ca2+、NO水平,改变cAMP和cGMP的水平,影响免疫系统的信号转导。Objective To study the effects of different concentrations of Allium Mongolicum Regel polysaccharides on the generation of signal molecules of sheep peripheral blood lymphocytes in vitro. Methods Alliurn Mongolicum Regel polysaccharides of different concentrations （0, 50, 100, 150, 200, 250μg/mL） were prepared and used to stimulate sheep peripheral blood lymphocytes cultured in vitro. Content of Ca2＋ in the media was tested by Fluo-3/AM fluorescence probe method and concentration of nitric oxide （NO） was measured by nitrate reductase. Contents of intracellular cyclic adenosine monophosphate （cAMP） and cyclic guanosine monophosphate （cGMP） were detected by ＇251 isotope labeled competitive protein combined with radioimmunoassay at 3,6, and 9 h after stimulation. Results High concentration of Allium Mongolicum Rege/polysaccharides significantly increased the concentration of Ca2＋ in sheep peripheral blood lymphocytes （P 〈 0.05）, and promoted the production of NO. Contents of cAMP and cGMP tended to decrease with the increasing concentration of A/lium Mongolicum Regel polysaccharides added into the media at 3h. At 6 and 9 h, concentrations of cAMP and cGMP in the media were significantly higher in 250 μg/mL stimulation group than in the control group （ P 〈 0.05）, however they did not alter in a regular tendency. Conclusion AI/ium Mongolicum Rege/polysaccharides could increase the concentrations of Ca2＋ and NO in peripheral blood lymphocytes, change the concentrations of cAMP and cGMP, thus affecting the signal transduction of immune system.

关 键 词：沙葱多糖 淋巴细胞 钙离子 环磷酸腺苷 环磷酸鸟苷 一氧化氮 

分 类 号：R392.11[医药卫生—免疫学] R285[医药卫生—基础医学]