慢性阻塞性肺病大鼠肺功能降低与Th1/Th2极化、STAT4/6表达的关系  

Relationship of reduced lung function with Th1/Th2 polarization, STAT4/6 expression in rats of chronic obstructive pulmonary disease

作　　者：王成阳[1] 李泽庚[2] 

机构地区：[1]湖北中医药大学研究生院,湖北武汉430056 [2]安徽中医药大学,安徽合肥230038

出　　处：《细胞与分子免疫学杂志》2013年第12期1233-1236,1241,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81072781)

摘　　要：目的研究慢性阻塞性肺病(COPD)大鼠Th1/Th2极化、转录信号转导子及转录激活子(STAT)蛋白表达及与肺功能的关系。方法将60只大鼠随机均分为空白组、假手术组、模型组。采用脂多糖(LPS)复制COPD大鼠模型,模型复制成功后28 d,检测大鼠肺功能变化,采用HE染色观察大鼠肺组织形态学变化,ELISA检测血清IFN-γ、IL-4、IL-12、IL-13变化,采用PCR法检测大鼠肺组织IFN-γmRNA、IL-4 mRNA的表达、Western blot法检测大鼠肺组织STAT4、STAT6蛋白表达。结果与空白组、假手术组比较,模型组大鼠肺组织炎性细胞浸润,血清IFN-γ、IL-12、Th1/Th2升高,肺功能和血清IL-4、IL-13降低;同时肺组织IFN-γmRNA和STAT4蛋白表达升高,IL-4 mRNA和STAT6蛋白降低(P<0.05或P<0.01)。相关性分析显示,COPD大鼠肺功能参数分别与IFN-γ、Th1/Th2、STAT4蛋白呈负相关,与IL-4、IL-13、IL-4 mRNA、STAT6蛋白呈正相关(P<0.01或P<0.05)。结论 COPD肺功能降低与气道炎症反应、Th1/Th2细胞失衡有关,STAT4、STAT6参与调控Th1/Th2细胞表达,共同导致COPD发生。Objective To study the correlations between lung function and Th1/Th2 polarization, signal transducer andactivator of transcription （STAT） proteins in rats of chronic obstructive pulmonary disease （COPD）. Methods Sixty ratswere randomly divided into normal control group, sham group and model group. The COPD rat models were developed bylipopolysaccharide （LPS）. The changes of pulmonary function were detected at 28 d after modeling. The lung tissues of ratswere observed by HE staining, and serum IFN-γ, IL-4, IL-12,and IL-13 were determined by ELISA. The mRNA expressionsof IFN-γ and IL-4 were examined by PCR, and STAT4,STAT6 proteins in lung tissues were detected by Western blotting. Results Inflammation of lung tissues was more serious,Jthe levels of serum IFN-γ, IL-12 and Th1/Th2, IFN-γ mRNA andSTAT4 protein in lung tissues were higher, and lung function, the levels of serum IL-4 and IL-13, IL-4 mRNA and STAT6protein in lung tissues were lower in model group than those in normal control and sham groups （P〈0. 05 or P〈0.01）_Correlation analysis showed that there were negative correlations between pulmonary function parameters and IFN-γ, Th1/Th2, STAT4 protein, and positive correlations between pulmonary function parameters and IL-4, IL-13,IL-4 mRNA, STAT6protein （P〈0.01 or P〈0.05）. Conclusion The reduced lung function was found related to airway inflammation, cell Th1/Th2imbalance in COPD. STAT4 and STAT6 involve in the regulation of Th1/Th2 cells. All of the above factors lead to theoccurrence of COPD in rats.

关 键 词：慢性阻塞性肺疾病 TH1细胞 TH2细胞 STAT4 STAT6 肺功能 

分 类 号：R392.11[医药卫生—免疫学] R392-33[医药卫生—基础医学]