Conjugated Polyelectrolyte-Ag＋ Fluorescent Switch for Biothiol Sensing  

作　　者：XIAO Yi LI Sha HUANG Hong-mei ZHANG Ye XUE Zun-zun TANG Xue-yan HE Xiao-xiao WANG Ke-min 

机构地区：[1]Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China [2]State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering College of Biology, Hunan University, Changsha 410082, P. R. China

出　　处：《Chemical Research in Chinese Universities》2013年第5期845-849,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（No.20805014）, the Scientific Research Fund of Hunan Pro- vincial Education Department, China（Nos. 11 C0809 and 12B077）, the Open Fund Project of Key Laboratory in Hunan University, China（No.201103） and the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province, China.

摘　　要：A new ＂off-on＂ switch for sensitive and selective fluorescence detection of biothiols[glutathione（GSH）, cysteine（Cys） and homocysteine（Hcy）] was developed based on an anionic conjugated polyelectrolyte（CPE）, pyridyl-functionalized poly（phenylene ethynylene）（Pl）. The fluorescence of P1 can be significantly quenched by Ag＋ due to complexation-mediated interpolymer aggregation. Furthermore, biothiols can efficiently recover the fluorescence intensity of P1 as a result of the stronger binding between thiol group and Ag＋, which dissociates PI from the P1/Ag＋ complex and disrupts interpolymer aggregation. Under optimum conditions, a good linear relation- ship in a range of 100--4200 nmol/L is obtained for GSH with a detection limit of 80 nmol/L（S/N=3）. As a result of specific interaction between the thiol group and Ag＋, the proposed method shows a high selectivity for biothiols. In addition, the CPE-based fluorescence ＂off-on＂ switch has been used to quantitatively detect total biothiols in cell lysates.A new ＂off-on＂ switch for sensitive and selective fluorescence detection of biothiols[glutathione（GSH）, cysteine（Cys） and homocysteine（Hcy）] was developed based on an anionic conjugated polyelectrolyte（CPE）, pyridyl-functionalized poly（phenylene ethynylene）（Pl）. The fluorescence of P1 can be significantly quenched by Ag＋ due to complexation-mediated interpolymer aggregation. Furthermore, biothiols can efficiently recover the fluorescence intensity of P1 as a result of the stronger binding between thiol group and Ag＋, which dissociates PI from the P1/Ag＋ complex and disrupts interpolymer aggregation. Under optimum conditions, a good linear relation- ship in a range of 100--4200 nmol/L is obtained for GSH with a detection limit of 80 nmol/L（S/N=3）. As a result of specific interaction between the thiol group and Ag＋, the proposed method shows a high selectivity for biothiols. In addition, the CPE-based fluorescence ＂off-on＂ switch has been used to quantitatively detect total biothiols in cell lysates.

关 键 词：Fluorescent switch Biothiol Silver（I） Conjugated polyelectrolyte 

分 类 号：O657.3[理学—分析化学] O647.2[理学—化学]