甘露寡糖对草鱼感染嗜水气单胞菌后细胞因子表达的调节  

Regulation of MOS on cytokines gene expression in grass carp infected with Aeromonas hydrophila

作　　者：刘佳佳[1] 吴志新[1] 石焱[1] 姚卓凤[1] 李景[1] 彭小云[1] 陈孝煊[1] 

机构地区：[1]华中农业大学水产学院水生动物医学系 农业部淡水生物繁育重点实验室 淡水水产健康养殖湖北省协同创新中心,武汉430070

出　　处：《淡水渔业》2013年第5期31-36,共6页Freshwater Fisheries

基　　金：国家科技支撑计划(2012BAD25B06;2012BAD25B05);公益性行业(农业)科研专项(200803013)

摘　　要：在饲料中添加0.2%甘露寡糖(Mannan oligosaccharide,(MOS)),饲养草鱼(Ctenopharyngodon idellus)28 d后,草鱼经腹腔注射3.2×106cell/mL的嗜水气单胞菌(Aeromonas hydrophila,Ah)0.1 mL,在注射嗜水气单胞菌48 h、72 h和96 h后,分别取草鱼头肾、脾脏和肝脏,利用荧光定量PCR分析甘露寡糖对草鱼感染嗜水气单胞菌后各组织中IL-1β和IL-10的表达影响。结果显示,在草鱼头肾中,MOS/I(0.2%甘露寡糖+注射Ah)组IL-1β的表达在注射嗜水气单胞菌后48 h和72 h显著高于CON/I(基础饲料+注射Ah)组;MOS/I组中1L-10的表达在注射嗜水气单胞菌72 h显著高于CON/I组,在96h时显著低于CON/I组。在草鱼脾脏中,MOS/I组IL-1β的表达在注射后72 h和96 h显著低于CON/I组;IL-10的表达在注射嗜水气单胞菌后48 h和96 h显著低于CON/I组。在草鱼肝脏中,MOS/I组IL-1β和IL-10的表达在注射嗜水气单胞菌后48 h显著高于CON/I组,但在注射后72 h时该2种基因的表达显著低于CON/I组。表明甘露寡糖在鱼体感染嗜水气单胞菌时能快速调节头肾、脾脏和肝脏中细胞因子的表达,增强鱼体抵抗细菌性病原的免疫力。The aim of the present study was to investigate the effect of MOS added in feeds on cytokines gene expression profile in grass carp （ Ctenopharyngodon idellus ） experimentally infected with Aeromonas hydrophila. After feeding C. idellus with Bio - Mos （MOS） and control （CON） diets for 28 days, gene expression of IL - 1β and IL - 10 were determined by real time - PCR. Head - kidney, liver and spleen were collected from infected （ n = 4） and non - infected （ n = 4 ） fish in 48 h, 72 h and 96 h. Our result showed MOS modulated the cytokines in innate immunity of C. ideUus, regulation varied in different tissue after injection. In head - kidney, gene expression of IL - 1β in MOS/I （ feed containing 2 g/kg Bio - Mos ＋ injection with Ah） group was significantly higher than CON/I （ non - supplemented feed ＋ injection with Ah） group in 48 h and 72 h; Gene expression of IL - 10 in MOS/I group was significantly higher than CON/I group in 72 h and was significantly lower than CON/I group in 96 h. In spleen, gene expression of IL - 1β in MOS/I group was significantly lower than CON/I group in 72 h and 96 h ; Gene expression of IL - 10 in MOS/I group was significantly lower than CON/I group in 48 h and 96 h. In liver, gene expression of IL - 10 and IL - 1 [3 in MOS/I group were significantly higher than CON/I group in 48 h, but lower than CON/I group in 72 h. These findings demonstrate that MOS induce host stimulatot7 responses which ultimately enhance disease resistance when pathogenie bacteria intruded.

关 键 词：草鱼(Ctenopharyngodon idellus) 甘露寡糖 IL-1β IL-10 

分 类 号：S948[农业科学—水产养殖]