市售氯硝柳胺在斑点叉尾组织中的残留消除规律研究  

Residue determination and degradation of niclosamide in Ictalarus puctatus tissue

作　　者：索纹纹[1,2] 刘永涛[1,3] 艾晓辉[1,3] 吕思阳[1] 杨秋红[1,3] 余少梅 

机构地区：[1]中国水产科学研究院长江水产研究所,农业部淡水鱼类种质监督检验测试中心,武汉430223 [2]湖南省水产科学研究所,长沙410153 [3]淡水健康养殖湖北省协调中心,武汉430223 [4]中博水产生物技术有限公司,武汉430207

出　　处：《淡水渔业》2013年第5期37-42,共6页Freshwater Fisheries

基　　金：公益性行业(农业)科研专项(201203085);农业行业标准项目(11555)

摘　　要：为了研究氯硝柳胺在斑点叉尾(Ictalurus punctalus)组织中的残留消除规律,在静水条件下经安全性试验确定安全浓度后,直接对水族箱泼洒25%氯硝柳胺乙醇胺盐(NES)粉剂后采集斑点叉尾皮肤、肌肉和水样。采用高效液相色谱-质谱联用法(HPLC-ESI/MS/MS)测定样品中氯硝柳胺的浓度,结果表明,静水条件下25%氯硝柳胺乙醇胺对斑点叉尾的安全浓度为0.113 mg/kg;养殖水环境中氯硝柳胺能快速消解,实验第2天即低于1μg/L,并呈无规律性持续较长时间;用药24 h内,氯硝柳胺在鱼体肌肉和鱼皮中具有较强的蓄积能力,但48 h后,其在鱼皮和肌肉中呈现快速消除至较低浓度,但后期消除缓慢,并会持续一定时间;氯硝柳胺(NIC)在斑点叉尾肌肉和鱼皮中的消除半衰期T1/2分别为10.66和9.24 d,显著大于在水体中的消除半衷期。Accumulation and elimination of Niclosamide （NIC） in Ictalurus punctatus tissue and water under indoor static water laboratory test were studied. We took the method of splashing 25% Niclosamide ethanolamine salt（NES） under the safe concentration into aquarium after the safety testing and the same sizes of I. punetaus （ 133.42±26. 2） g were exposed to freshwater containing NES under static conditions. The concentrations of NIC in water and tissue （muscle, skin） samples were determined by liquid chromatography tandem mass spectrometric （HPLC-ESI/MS/MS）. The results showed that the safe concentration of 25% NES on 1. Punetaus was 0. 113 mg/L. NIC concentration in water decreased quickly on the first day, then it was lower than 1 p^g/L and maintained in a certain period, but the NIC concentration in muscle and skin presented enriched obviously on the first day, then the elimination trends started to level off and maintained a certain concentration fraction in a certain period. The elimination half-life of NIC in muscle and skin were 10. 66 and 9. 24, respectively, which were significantly higher than that in water.

关 键 词：氯硝柳胺 斑点叉尾(鱼回)(Ictalurus punctalus) 消解 残留 (鱼回) 

分 类 号：S948[农业科学—水产养殖]