两株光合细菌的分离鉴定及其水质净化效果研究  

Selection and identification of two photosynthetic bacteria strains and their purification effect for aquaculture wastewater

作　　者：刘洋[1] 舒巧玉[1] 楼春燕[1] 吴湘[1] 韩志萍[1] 叶金云[1] 

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000

出　　处：《淡水渔业》2013年第5期57-61,共5页Freshwater Fisheries

基　　金：国家水体污染控制与治理科技重大专项(2011ZX07101-012-008);现代农业产业技术体系建设专项资金(CARS-46-21);浙江省重大科技专项重大农业项目(2010C02001);浙江省重点科技创新团队项目(2012R10026-14);湖州市重大科技专项(2010ZD1002);湖州市自然科学资金项目(2010YZ08)

摘　　要：为了筛选能够高效净化水质的光合细菌菌株,采用半固体试管法和双层平板法,从市售光合菌菌液中分离得到2株光合细菌,分别命名为GHJ-1、GHJ-2,对菌株进行了形态学观察、生理生化及16S rDNA鉴定,比较了这2株光合细菌对青鱼(Mylopharyngodon piceus)养殖污水的净化效果。结果表明:经鉴定这两株光合细菌为Rhodovulum sp菌株。菌株GHJ-1、GHJ-2对污水进行15 d的净化处理后,硝酸盐去除率分别为91.4%和96.3%;亚硝酸盐的去除率分别为68.9%和82.7%;氨氮去除率分别为56.8%和67.5%;COD Mn的去除率分别为61.7%和42.4%。In order to select the photosynthetic bacteria （PSB） strains to be used for aquaculture wastewater purification, two PSB strains, named GHJ-1, GHJ-2, were isolated from commercial PSB broth by anaerobic culture, semi-solid tube and double-layer plate culture methods. The two obtained strains were investigated based on morphology, physio-biochemi- cal characteristics and 16S rDNA sequence analysis. Their purification effects for the effluent from pond of Mylopharyng- odon piceus culture were determined in the experiment. Both of the strains GHJ-1 and GHJ-2 were identified as Rhodovu- lure sp. The removal rates for nitrate, nitrite, ammonia nitrogen and chemical oxygen demand （COD） from the pond ef- fluent, by strains GHJ-1 and GHJ-2, were 91.4% and 96. 3% , 68.9% and 82. 7% , 56. 8% and 67.5% , 61.7% and 42.4% , respectively.

关 键 词：光合细菌 分离鉴定 青鱼(Mylopharyngodon piceus) 养殖污水 净化 

分 类 号：S965.89[农业科学—水产养殖]