胀果甘草药渣总黄酮和甘草查尔酮A的制备及其体外抗肿瘤活性研究  

Preparation and antitumor activities in vitro of total flavonoids from residues of Glycyrrhiza inflata and licochalcone A

作　　者：韩龙哲[1] 张娟 倪慧 刘芬[3] 李宁[3] 贾晓光 

机构地区：[1]延边大学附属医院病理科,吉林延吉133000 [2]新疆维吾尔自治区中药民族药研究所,新疆乌鲁木齐830002 [3]沈阳药科大学中药学院基于靶点的药物设计与研究教育部重点实验室,辽宁沈阳110016

出　　处：《现代药物与临床》2013年第5期668-672,共5页Drugs & Clinic

基　　金：新疆维吾尔自治区科技支撑计划项目(201333102);新疆维吾尔自治区卫生厅青年科技人才专项科研项目(2012Y06)

摘　　要：目的研究胀果甘草药渣总黄酮和其指标性成分甘草查尔酮A的制备及其体外抗肿瘤活性。方法利用大孔树脂柱色谱、聚酰胺柱色谱等方法,制备甘草药渣总黄酮,并结合色谱法和波谱法分离鉴定指标性甘草查尔酮A,应用HPLC法测定了总黄酮中甘草查尔酮A。应用A549、H1792、Calu-1 3种人癌细胞系,采用MTT法和流式细胞术法系统评价甘草药渣总黄酮和甘草查尔酮A的体外抗肿瘤活性和作用机制。结果制备得到甘草药渣总黄酮,并从中分离鉴定了特征性指标性成分甘草查尔酮A,测定其在甘草药渣总黄酮中质量分数为7.41%。甘草药渣总黄酮和甘草查尔酮A对A549、H1792、Calu-1人癌细胞系均具有显著的抑制活性,可诱导肿瘤细胞凋亡。经流式细胞术检测,推测其作用机制为诱导肿瘤细胞凋亡。结论甘草查尔酮A为胀果甘草药渣总黄酮发挥体外抗肿瘤活性的有效成分。Objective To prepare the total flavonoids and its characteristic constituent licochalcone A from the residues of Glycyrrhiza inflata,which were further assayed for the antitumor activities in vitro.Methods The total flavonoids and licochalcone A were purified by chromatography.The structure of licochalcone A was identified on the basis of spectral data.The concentration of licochalcone A was determined by HPLC analysis method.Using MTT and flow cytometry,the antitumor activities in vitro were tested against A549,H1972,and Calu-1 human cancer cell lines.Results The total flavonoids and licochalcone A were prepared respectively and the concentration of licochalcone A was determined as 7.41% in the total flavonoids.The total flavonoids and licochalcone A showed the significant antitumor activities against the above three cancer cell lines.Its possible mechanism was the induction of apoptosis.Conclusions Licochalcone A is the effective antitumor constituent of the total flavonoids from the residues of G.inflata.

关 键 词：胀果甘草药渣总黄酮 甘草查尔酮A 体外抗肿瘤活性 细胞凋亡 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]