HPLC法测定肾上腺素和去甲肾上腺素原料药中有关物质  

Determination of related substances in adrenaline and noradrenaline crude drugs by HPLC

作　　者：李佩[1] 李银峰[2] 王杰晶[3] 张园园[1] 靳朝东[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津药物研究院分析测试中心,天津300193 [3]河南大学,河南开封475001

出　　处：《现代药物与临床》2013年第5期720-723,共4页Drugs & Clinic

摘　　要：目的建立肾上腺素、去甲肾上腺素原料药中有关物质的HPLC分析方法。方法采用五氟苯基柱(250 mm×4.6mm,5μm),流动相为0.03 mol/L磷酸二氢铵–甲醇(98∶2),检测波长280 nm,进样量20μL,体积流量1.0 mL/min,柱温20℃。结果肾上腺素、去甲肾上腺素两种物质与其降解产物均能达到基线分离,重复性好,且呈现较好的线性关系。结论该方法操作简单、快速、灵敏度高,可用于肾上腺素、去甲肾上腺素原料药中有关物质的测定。Objective To establish an HPLC method for the determination of related substances in adrenaline and noradrenaline crude drugs.Methods The samples were performed on PFP column（250 mm × 4.6 mm,5 μm） with the mobile phase of 0.03 mol/L ammonium dihydrogen phosphate-methanol（98∶2）.The detection wavelength was 280 nm and column temperature was 20 ℃ with injection volume of 20 μL at a flow rate of 1.0 mL/min.Results Two components adrenaline,noradrenaline and their degradation products were well separated with good repeatability and linearity.Conclusion The method is simple,rapid,and sensitive,which could be applied for the determination of the related substances in adrenaline and noradrenaline crude drugs.

关 键 词：肾上腺素 去甲肾上腺素 有关物质 高效液相色谱 

分 类 号：R927.1[医药卫生—药学]