HPLC法测定乳痈消痛片中熊果酸  

Determination of ursolic acid in Ruyong Xiaotong Tablet by HPLC

作　　者：刘茂军[1] 韩锋[2,3] 高静[2,3] 裴静[4] 马莉[2,3] 

机构地区：[1]天津市和平区中医医院,天津300050 [2]天津药物研究院,天津300193 [3]中药现代制剂与质量控制技术国家地方联合工程实验室(天津),天津300193 [4]天津中医药大学,天津300193

出　　处：《现代药物与临床》2013年第5期727-729,共3页Drugs & Clinic

摘　　要：目的建立乳痈消痛片中熊果酸的HPLC测定方法。方法采用高效液相色谱法,Kromasil C18色谱柱（250 mm×4.6 mm,5μm）;流动相：甲醇–2%醋酸水溶液（90∶10）;体积流量：1.0 mL/min;柱温：35℃;检测波长：210 nm。结果熊果酸在0.506-4.554μg线性关系良好（r=1.000）,平均加样回收率为99.95%,RSD值为0.98%（n=9）。结论该方法灵敏度高、准确、快速、专属性强、重复性好,可用于乳痈消痛片中熊果酸的质量控制。Objective To establish an HPLC method for determination of ursolic acid in Ruyong Xiaotong Tablet.Methods Ursolic acid was determined on Kromasil C18 column（250 mm × 4.6 mm,5 μm） with methanol-2% acetic acid（90∶10） as mobile phase.The flow rate was 1.0 mL/min.The column temperature was 35 ℃,and the detection wavelength was 210 nm.Results The ursolic acid showed a good linear relationship in the range of 0.506—4.554 μg（r = 1.000）.The average recovery of ursolic acid was 99.95% with RSD of 0.98%（n=9）.Conclusion This method is accurate,fast,reliable,reproducible,and suitable for the determination of ursolic acid in Ruyong Xiaotong Tablet.

关 键 词：乳痈消痛片 熊果酸 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]