鞘氨醇单胞菌TP-3中基因spsK的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of spsK Gene from Sphingomonas sanxanigenens sp. nov. TP-3

作　　者：黄海东[1] 詹欣娜[1] 李娜[1] 

机构地区：[1]天津农学院农学系,天津300384

出　　处：《天津农学院学报》2013年第3期1-4,共4页Journal of Tianjin Agricultural University

基　　金：天津市自然科学基金重点项目"新型鞘氨醇胶合成关键基因的研究"(11JCZDJC16600)

摘　　要：以自杀性质粒pUT携带的mini-Tn5转座子对菌株TP-3进行转座诱变,从转座突变库中筛选到不能合成产物鞘氨醇胶Ss的突变株,进而采用Tn5外向扩增与LP-RAPD相结合的方法,得到糖基转移酶编码基因spsK的801 bp序列全长。生物信息学分析表明,其氨基酸序列与迪特胶以及S88合成途径中的糖基转移酶同源性最高,达47%;SpsK蛋白具有典型的G-loop和DXD保守模式,属于糖基转移酶的GT-A家族。Strian TP-3 was induced by transposon mini-Tn5 on suicide plasmid pUT, and a mutant strain which could not produce sphingan Ss was screened. The spsK gene of TP-3 was obtained by the method of Tn5 flanking PCR and LP-RAPD. The spsK gene coding region includes 801 bp and encodes glycosyl transferase. The bioinformatics analysis reveals that spsK from strain TP-3 showed the highest of 47% amino acid sequence identity with glycosyl transferase from biosynthesis of diutan and S88 sphingan. SpsK protein from strain TP-3 belongs to the glycosyl transferase GT-A family with the typical motif of G-loop and DXD.

关 键 词：鞘氨醇单胞菌 鞘氨醇胶Ss 糖基转移酶 生物信息学 

分 类 号：Q781[生物学—分子生物学]