高原低氧对大鼠下丘脑谷氨酸、天门冬氨酸和NOS的影响  

Effect of altitude hypoxia on glutamate, aspartate and NOS in the rat hypothalamus

作　　者：阮怀珍[1] 范晓棠[1] 张金海[1] 袁碧波[1] 吴喜贵[1] 

机构地区：[1]第三军医大学神经生物学教研室,重庆400038

出　　处：《中国病理生理杂志》2000年第11期1198-1201,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目! (No 396 70 2 83)

摘　　要：目的 :观察高原低氧大鼠下丘脑谷氨酸 (Glu)、天门冬氨酸 (Asp)和一氧化氮合酶 (NOS)的变化。方法 :应用氨基酸测定和NADPH -d组化法 ,检测高原低氧模型大鼠下丘脑Glu、Asp含量和NADPH -d阳性神经元的数量。结果 :高原低氧大鼠下丘脑Glu、Asp含量明显增多 ,室旁核、视上核可见密集深染的NADPH -d阳性神经元 ;用NMDA受体拮抗剂氯氨酮 (Ketamine)和AP -V对高原低氧大鼠进行预处理后置于低压氧舱 ,观察到大鼠下丘脑室旁核、视上核NADPH -d阳性神经元数明显少于相应时间的高原低氧组 (P <0 0 1)。结论 :NMDA受体可能参与了高原低氧引起的下丘脑NOS的表达。AIM: To observe the effect of altitude hypoxia on glutamate, aspartate and nitric oxide synthase (NOS) in the rat hypothalamus. METHODS: Using altitude hypoxia model, amino acid analysis system and the NADPH-d histochemistry, we determined the content of glutamate, aspartate and the number of NADPH-d neurons in the rat hypothalamus. RESULTS: After altitude hypoxia, the contents of glutamate, aspartate in the hypothalamus of rats were increased significantly, densely and deeply stained NADPH-d neurons were seen in hypothalamic paraventricular nucleus (PVN)and supraoptic nucleus(SON). If rat were pretreated with the NMDA receptor blockers Ketamine (ip,40 mg/Kg)or AP-V(i.c.v, 10 μg) , the number of NADPH-d neurons in the rat hypothalamic PVN and SON was markedly less than that in corresponding altitude hypoxia group. CONCLUSION: NMDA receptor may take part in the expression of hypothalamic NOS induced by altitude hypoxia.

关 键 词：下丘脑 高原低氧 谷氨酸 天门冬氨酸 NOS 

分 类 号：R339.54[医药卫生—人体生理学] R338.27[医药卫生—基础医学]