利用高效液相色谱法检定甜高粱茎秆糖分的方法研究  

STUDY ON EXTRACTING SUGAR OF SWEET SORGHUM STALK WITH HPLC

作　　者：丛靖宇[1] 刘伟 张新[1] 张飞[1] 王瑞刚[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]乌海市林业技术推广中心,乌海0150000

出　　处：《内蒙古农业大学学报（自然科学版）》2013年第4期80-85,共6页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：农业科技成果转化资金项目(2012GB2A400069);内蒙古自然基金项目(2011MS0307)

摘　　要：甜高粱是重要的能源作物,然而,甜高粱综合利用技术还非常有限,研究甜高粱茎秆糖分组成及其代谢规律是进一步发展甜高粱产业的理论基础,为优化甜高粱茎秆可溶性糖提取工艺,建立高效液相色谱检测方法以及阐明甜高粱糖代谢机理提供依据和参考。本研究通过L9(33)正交试验,研究了温度、提取时间、料液比对甜高粱茎秆可溶性糖提取的影响。结果表明:温度和提取时间是影响甜高粱茎秆可溶性糖提取率的主要因素,其最佳工艺为70℃下,以1:20的料液比提取1h。Sweet sorghum, having a rapid growth rate and more significant yield potential, is known as ＂high energy crop＂. Developing sweet sorghum is significant in maintaining the safety of energy, food and environment in consideration of the exploitation of bio - energy made from tire resource of no - maple food nowadays. Our research is aimed to reveal the sweet sorghum extraction of soluble sugar, establishing its sugar HPLC detection method and elucidating the mechanism of glucose metabolism in sweet sorghum. By the L9 （33） orthogonal design, the effect of temperature, extraction time, and solid -liquid ratio on the soluble sugar extraction efficiency were studied. The results showed ： temperature and extraction time are the major factors that affected soluble sugar extraction rate, and tire optimum condition is extracting one hour at 70℃ with solid - liquid ratio of 1：20.

关 键 词：甜高粱 可溶性糖 正交设计 提取工艺 高效液相色谱 

分 类 号：S548[农业科学—作物学]