连作对作物根际土壤微生物菌群及酶活性影响  

Effects of Continuous Cropping on Soil Microbe Communities and Enzyme Activity

作　　者：乔蓬蕾[1] 吴凤芝[1] 周新刚[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030

出　　处：《沈阳农业大学学报》2013年第5期524-530,共7页Journal of Shenyang Agricultural University

基　　金：国家重点基础研究发展计划"973"专题项目(2009CB119004-05);黑龙江省教育厅科学技术研究项目(12531029);黑龙江省自然科学基金项目(C201028)

摘　　要：连作障碍是制约农业生产可持续发展亟待解决的问题,造成连作障碍的原因很多,而土壤微生物菌群及土壤酶活性的变化对于土壤健康有至关重要的作用,被认为是连作障碍的主要因子。围绕连作引起的作物土壤酶活性、土壤微生物数量及群落结构以及特异微生物的变化,提出将高通量测序技术及基因芯片技术与传统分子生物学技术相结合,重点研究微生物菌群活性和功能变化以及菌群之间的相互作用对连作障碍的影响,同时对从作物根系分泌物对土壤微生物菌群影响的角度和微生物细胞水平解决连作障碍提出了展望。旨在为农业生态系统的可持续发展提供参考。Continuous cropping obstacle restricts sustainable development of agricultural production, which is an important issue that needs to be solved urgently. It is caused by many factors. The variation of soil microbe communities and enzyme activity that plays an important role for soil health was considered the main factors. This article focuses on the variation of soil activity of soil enzyme, microorganism population and community composition and specific nficroorganism caused by continuous cropping. Additionally, it was proposed that effects of the variation of microbe eonsortiums activity and function and interaction among then] on continuous cropping obstacle should be studied as key research using high-throughput sequencing technology, functional gene microarrays and traditional molecular biology techniques. Meanwhile, it was suggested that continuous cropping obstacle might be solved through the impact of root exudates on soil microflora and microbial cells. The objective was to supply reference for sustainable development of agricultural ecosystems.

关 键 词：连作障碍 微生物 土壤酶 群落结构 分子生物学 高通量测序技术 基因芯片技术 

分 类 号：S344.5[农业科学—作物栽培与耕作技术] S154.74[农业科学—农艺学]