抗心磷脂抗体检测及其影响因素的研究  

Studies on affected factors about antipholipid antibodies determinations

作　　者：裴瑾[1] 米东辉[2] 曲立科[1] 杨翰仪[1] 

机构地区：[1]白求恩医科大学基础医学院生化与分子生物学教研室,长春130021 [2]白求恩医科大学第三临床学院呼吸内科,长春130041

出　　处：《中国免疫学杂志》2000年第11期598-600,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金!资助项目 (395 70 317)

摘　　要：目的 :研究血清样品稀释度、温度、离子强度以及样品检测间隔时间对血清抗心磷脂抗体 (ACA)检测结果的影响。方法 :采用酶联免疫吸附测定法 (ELISA)检测ACA。结果 :血清样品最佳稀释度为 1∶5 0 ;37℃时光吸收值比 2 2℃和 2 5℃的光吸收值显著下降 ;15mol LNaCl可明显抑制ACA与抗原结合 (P <0 0 1) ,抑制率平均为 45 4% ;包被 14d以上的检测结果明显高于 1d和 7d的测定结果 (P <0 0 5 )。结论 :血清ACA检测结果受血清样品稀释度、温度、离子强度及包被时间影响。Objective:Human sera ACA was detected by ELISA method and some factors including the dilution rate of sera, blocking materials, temperature,ionic strength and interval time between blocking and detection,which could probablly influence the detective level of sera ACA were investigated.Methods:Human sera ACA was detected by ELISA.Results:10% NBS/PBS was more feasible than 1% bovine albumin/PBS for blocking non specific binding;the ACA titer of the same sample detected at 37℃ was lower than that of 22℃ and 25℃,the dilution rate 1∶50 for sera was suitable for the detection; time internal between blocking and detection could influence ACA level when it exceeding 2 weeks;the blinding ability of Ag with Ab was signicantly inhibited when the NaCl inonic strength was higher than 1.5 mol/L.Conclusion:The detected results of human sera ACA influenced by the dilution rate of sera,blocking materials,temperature ionic strength and internal time between blocking and detection.

关 键 词：抗心磷脂抗体 酶联免疫吸附法 系统性红斑狼疮 

分 类 号：R593.241[医药卫生—内科学] R392.11[医药卫生—临床医学]