丙型肝炎病毒与恶性疟原虫复合多表位抗原基因在小鼠及家兔中免疫应答的研究  

Immunogenicity of a hybrid multiple epitopes antigen gene of hepatitis C virus and P.falciparum in mice and rabbits

作　　者：黄建生[1] 解咏梅[2] 张潜[2] 沈先荣 钟雄林[1] 张丽芸[1] 郭明秋[1] 任大明[2] 

机构地区：[1]第一军医大学抗衰老研究室,广州510515 [2]复旦大学生命科学院,上海200433

出　　处：《中国免疫学杂志》2000年第10期533-534,538,共3页Chinese Journal of Immunology

摘　　要：目的 :探讨丙型肝炎病毒 (HCV)及恶性疟原虫 (Pf)复合DNA疫苗的可行性。方法 :把HCV复合多表位抗原基因PCX与Pf复合基因AB克隆到带CMV启动子的真核表达载体pcDNA3中 ,构建真核表达载体pcDNA3 CAB ,肌肉注射免疫小鼠及家兔 ,检测其诱发特异性免疫应答水平及安全性。结果 :小鼠及家兔分别于免疫后第 6周及第 8周可检测到抗GZ PCX抗体 ,于第 10周达最高 ,滴度分别为 1:40 0及 1:3 2 0 0 ,但持续时间较短 ;免疫血清可识别Pf抗原 ;免疫动物还可产生针对GZ PCX融合蛋白的迟发性超敏反应 ;免疫后小鼠体重正常 ,肝脾脏未见明显肿大 ,具有良好的安全性。结论 :HCV Pf双价多表位DNA抗原基因在小鼠及家兔中可诱发特异性免疫应答 ,但抗体的持续时间较短。Objective:To explore the possibility of a bivalent DNA vaccine specific for hepatitis C virus (HCV) and Plasmodium falciparum(Pf).Methods:Two synthetic multiple epitopes antigen gene PCX of HCV and AB of Pf were cloned into eukaryotic vector pcDNA3 with CMV promoter to construct a HCV/Pf eukaryotic expression vector pcDNA3/CAB.Humoral and cellular immunity and safety were studied after the plasmid was genetically immunized mice and rabbits.Results:Specific antibodies against GZ-PCX antigen were detected at week 6th and 8th in mice and rabbits and the highest titers of anti-GZ-PCX IgG were 1:400 and 1:3 200,respectively.The immunized antisera also recognized Pf antigen.Delayed type hypersensitivity (DTH)reactions were induced by GZ-PCX protein.The body weight immunized mice and rabbit were normal and no obvious toxicity appeared.Conclusion:The study shows that the HCV/Pf hybrid antigen gene induced specific immune responses and might be able to provide some data for the researches of bivalent vaccine against HCV and Pf.

关 键 词：丙型肝炎病毒 恶性疟原虫 多表位抗原 DNA疫苗 

分 类 号：R512.63[医药卫生—内科学] R531.3[医药卫生—临床医学]