PEG修饰的腺病毒-阴离子脂质体复合物的制备及分离纯化  

Preparation and purification of PEG modified adenovirus-anionic liposomes complexes

作　　者：万瑜[1] 樊戈睿 郑洁[1] 孙逊[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2013年第5期444-446,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家科技重大专项[编号:2011ZX09401-304(4-3)]

摘　　要：目的制备并分离纯化PEG修饰的腺病毒-阴离子脂质体复合物。方法采用薄膜分散法制备空白阴离子脂质体,利用钙离子融合法制备阴离子脂质体与腺病毒的复合物,最后用后插入法制备PEG修饰的腺病毒-阴离子脂质体复合物。通过蔗糖密度梯度离心法分离制备的复合物,分别测定分离得到的各组分中脂质体、PEG-脂质材料及腺病毒的含量。结果成功制备了PEG修饰的腺病毒-阴离子脂质体复合物,连接到脂质体上的PEG-脂质占加入总量的28.9%,被脂质体包裹的腺病毒占加入量的35.9%。结论通过蔗糖密度梯度离心可得到纯化的PEG修饰的腺病毒-阴离子脂质体复合物。OBJECTIVE To prepare and purify the PEG modified adenovirus - anionic liposomes complexes. METHODS Blank anionic liposomes were prepared by the thin film hydration and sonieation dispersion technique. A calcium - induced phase change method was applied to encapsulate adenovirus 5 （ Ad5 ） into anionic liposomes to formulate the complexes of AdS and anionic liposomes （AL -AdS ）. And then the PEG modified adenovirus -anionic liposomes complexes（ PPC -AL- AdS ） were constructed by the post - insertion method. The PPC - AL - AdS complexes were purified by sucrose density gradient centrifugation. The contents of liposomes, PEG - Lipids and adenovirus in each fraction were determined as well. RESULTS The PPC - AL - AdS complexes were prepared successfully. PEG - Lipids accounting for 28.9% of the total adding were conjugated to the liposomes, and adenovirus accounting for 35.9% of the total adding were encapsulated by the liposomes. CONCLUSION The purified PPC - AL - AdS complexes could be obtained by the sucrose density zradient centrifugation.

关 键 词：PEG 腺病毒 阴离子脂质体 蔗糖密度梯度离心 

分 类 号：R94[医药卫生—药剂学]