人参皂苷Rb1抗血管平滑肌细胞增殖与calcineurin通路及抗氧化作用的关系  

The anti-proliferative effect of ginsenoside Rb1 on vascular smooth muscle cells and its relationship with calcineurin signaling and anti-oxidant effect

作　　者：张纾[1,2] 高杨[1] 邓江[1] 黄燮南[1] 

机构地区：[1]遵义医学院药理教研室贵州省基础药理重点实验室,贵州遵义563000 [2]福建省药品不良反应监测中心,福建福州350001

出　　处：《华西药学杂志》2013年第5期461-463,共3页West China Journal of Pharmaceutical Sciences

基　　金：贵州省优秀科技教育人才省长专项基金项目(编号:2007-53)

摘　　要：目的观察人参皂苷Rb1(Rb1)抗血管平滑肌细胞增殖效应与钙调神经磷酸酶(CaN)信号通路的关系,并探讨该药抗增殖效应是否与其抗氧化作用有关。方法采用球囊导管损伤血管内皮造成大鼠颈动脉内膜增生,ip Rb1两周后,取血样测定过氧化物歧化酶(SOD)活性及丙二醛(MDA)含量变化;在培养的血管平滑肌细胞(VSMC)中加入血小板衍化生长因子-BB(PDGF-BB)诱导其增殖,给予Rb1 24 h后,观察VSMCs增殖和CaN mRNA表达的改变。结果 10、30 mg·kg-1·d-1Rb1给药两周使球囊损伤而变窄的大鼠颈动脉管腔面积明显扩大,伴有SOD活性升高和血中MDA水平的降低;在培养VSMCs中,加入抗增殖浓度Rb1(25、50、100μg·mL-1)后,显著下调PDGF-BB所致CaN mRNA的过度表达。结论 Rb1抗VSMC增殖效应至少部分与其抗氧化作用有关,并可能涉及其对CaN通路的抑制。OBJECTIVE To investigate whether the anti - proliferative effect of ginsenoside Rbl （ Rbl ） on vascular smooth muscle cells （VSMCs） was involved in the inhibition of ealeineurin （CaN） signaling and its anti - oxidant action. METHODS The carotid neointimal hyperplasia model of rat was induced by rubbing the endothelia of vessel with a balloon, and the plasma superoxide dismutase （SOD） activity and malondialdehyde （MDA） eontant were detected after Rbl -administration（ip） two weeks later. The platelet derived growth factor- BB（ PDGF -BB） was added in cultural VSMCs to elicit proliferation,and the CaN mRNA expression was exam- ined by real time RT - PCR at 24 h after Rbl - administration. RESULTS Administration of Rbl （ 10,30 mg· kg- l · d - 1 ） for two weeks could widen the narrowed lumen of the rat carotid artery induced by balloon - injury, with increase SOD activity and decrease MDA eontant in the plasma. Rbl at the concentrations that could inhibit the cultural VSMC proliferation and also could down - regulate the CaN mRNA expression by PDGF - BB. CONCLUSION The anti - proliferative effect of Rbl on VSMC is partly related to its anti -oxidant action and may be involved in the inhibition on CaN signaling.

关 键 词：人参皂苷RB1 血管平滑肌细胞增殖 钙调神经磷酸酶信号通路 抗氧化作用 

分 类 号：R96[医药卫生—药理学]